|
Status |
Public on Feb 12, 2019 |
Title |
APA4_S9_L001_R1_001 |
Sample type |
SRA |
|
|
Source name |
aldosterone-producing adenoma
|
Organism |
Homo sapiens |
Characteristics |
tissue: plasma method: EDTA-anticoagulated plasma
|
Extracted molecule |
total RNA |
Extraction protocol |
EDTA-anticoagulated plasma samples were isolated by QIAGEN miRNeasy Serum/Plasma Kit Library was made by QIAseq microRNA Library Kit (by Qiagen)
|
|
|
Library strategy |
miRNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina MiSeq |
|
|
Data processing |
trimming of adapters using cutadapt (http://cutadapt.readthedocs.io/en/stable/guide.html). Reads with less than 16 bp insert sequences or with less than 10 bp Unique Molecular Index (UMI) were discarded. Alignment of reads was performed using bowtie (http://bowtie-bio.sourceforge.net/index.shtml), miRbase V21 was used for miRNAs. Genome_build: miRbase V21 Supplementary_files_format_and_content: .xls file was generated by Qiagen online primary data analysing tool: (http://ngsdataanalysis.sabiosciences.com/QIAseqmiRNA).
|
|
|
Submission date |
Feb 11, 2019 |
Last update date |
Feb 12, 2019 |
Contact name |
Abel Decmann |
E-mail(s) |
decabel@gmail.com
|
Organization name |
Semmelweis University
|
Department |
Department of Internal Medicine and Oncology
|
Lab |
Endocrine Genetics
|
Street address |
Korányi str. 2a
|
City |
Budapest |
ZIP/Postal code |
1083 |
Country |
Hungary |
|
|
Platform ID |
GPL15520 |
Series (1) |
GSE126386 |
Circulating miRNA expression profiling in primary aldosteronism |
|
Relations |
BioSample |
SAMN10910558 |
SRA |
SRX5359085 |