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Sample GSM3597582 Query DataSets for GSM3597582
Status Public on Feb 12, 2019
Title apa9_S9_L001_R1_001
Sample type SRA
 
Source name aldosterone-producing adenoma
Organism Homo sapiens
Characteristics tissue: plasma
method: EDTA-anticoagulated plasma
Extracted molecule total RNA
Extraction protocol EDTA-anticoagulated plasma samples were isolated by QIAGEN miRNeasy Serum/Plasma Kit
Library was made by QIAseq microRNA Library Kit (by Qiagen)
 
Library strategy miRNA-Seq
Library source transcriptomic
Library selection size fractionation
Instrument model Illumina MiSeq
 
Data processing trimming of adapters using cutadapt (http://cutadapt.readthedocs.io/en/stable/guide.html).
Reads with less than 16 bp insert sequences or with less than 10 bp Unique Molecular Index (UMI) were discarded.
Alignment of reads was performed using bowtie (http://bowtie-bio.sourceforge.net/index.shtml),
miRbase V21 was used for miRNAs.
Genome_build: miRbase V21
Supplementary_files_format_and_content: .xls file was generated by Qiagen online primary data analysing tool: (http://ngsdataanalysis.sabiosciences.com/QIAseqmiRNA).
 
Submission date Feb 11, 2019
Last update date Feb 12, 2019
Contact name Abel Decmann
E-mail(s) decabel@gmail.com
Organization name Semmelweis University
Department Department of Internal Medicine and Oncology
Lab Endocrine Genetics
Street address Korányi str. 2a
City Budapest
ZIP/Postal code 1083
Country Hungary
 
Platform ID GPL15520
Series (1)
GSE126386 Circulating miRNA expression profiling in primary aldosteronism
Relations
BioSample SAMN10910552
SRA SRX5359090

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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