|
Status |
Public on Nov 04, 2019 |
Title |
Zfp281_sh10_Myc |
Sample type |
SRA |
|
|
Source name |
murine embryonic stem cells
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 (female) x 129/S (male) cell type: murine embryonic stem cells chip antibody: C-Myc(N-262) (Santa Cruz, catalog# sc-764, lot# 12613)
|
Growth protocol |
Mouse embryonic stem cells V6.5 were cultured on irradiated mouse embryonic fibroblast (MEF) feeder layers in 0.1% gelatin-coated tissue culture flask.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
For ChIP-Seq, 5×10^7 cells were used per immunoprecipitation according to previously described protocols (Lee et al., 2006). ChIP-Seq libraries were prepared with Illumina's ChIP-Seq sample prep kit (0801-0303). NEBNext® DNA Library Prep Master Mix Set for Illumina
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
ChipSeq samples were aligned using Bowtie2 (v2.2.5) allowing uniquely mapping reads only Peak calling was performed with MACS2 (v2.1.1) and filtered at FDR < 5 (0.05) Genome_build: mm9 Supplementary_files_format_and_content: bigwig
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|
|
Submission date |
Feb 20, 2019 |
Last update date |
Nov 05, 2019 |
Contact name |
Xiaoxu Liu |
E-mail(s) |
liuxiaoxu1010@126.com
|
Organization name |
Southeast University
|
Department |
Institute of Life Sciences
|
Lab |
the Key Laboratory of Developmental Genes and Human Disease
|
Street address |
2 Sipailou
|
City |
Nanjing |
State/province |
Jiangsu |
ZIP/Postal code |
210018 |
Country |
China |
|
|
Platform ID |
GPL9185 |
Series (1) |
GSE125435 |
ZFP281 recruits MYC to active promoters in regulating transcriptional initiation and elongation |
|
Relations |
BioSample |
SAMN10970972 |
SRA |
SRX5394949 |