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Sample GSM3613354 Query DataSets for GSM3613354
Status Public on Nov 04, 2019
Title Zfp281_sh10_Myc
Sample type SRA
Source name murine embryonic stem cells
Organism Mus musculus
Characteristics strain: C57BL/6 (female) x 129/S (male)
cell type: murine embryonic stem cells
chip antibody: C-Myc(N-262) (Santa Cruz, catalog# sc-764, lot# 12613)
Growth protocol Mouse embryonic stem cells V6.5 were cultured on irradiated mouse embryonic fibroblast (MEF) feeder layers in 0.1% gelatin-coated tissue culture flask.
Extracted molecule genomic DNA
Extraction protocol For ChIP-Seq, 5×10^7 cells were used per immunoprecipitation according to previously described protocols (Lee et al., 2006). ChIP-Seq libraries were prepared with Illumina's ChIP-Seq sample prep kit (0801-0303).
NEBNext® DNA Library Prep Master Mix Set for Illumina
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer
Data processing ChipSeq samples were aligned using Bowtie2 (v2.2.5) allowing uniquely mapping reads only
Peak calling was performed with MACS2 (v2.1.1) and filtered at FDR < 5 (0.05)
Genome_build: mm9
Supplementary_files_format_and_content: bigwig
Submission date Feb 20, 2019
Last update date Nov 05, 2019
Contact name Xiaoxu Liu
Organization name Southeast University
Department Institute of Life Sciences
Lab the Key Laboratory of Developmental Genes and Human Disease
Street address 2 Sipailou
City Nanjing
State/province Jiangsu
ZIP/Postal code 210018
Country China
Platform ID GPL9185
Series (1)
GSE125435 ZFP281 recruits MYC to active promoters in regulating transcriptional initiation and elongation
BioSample SAMN10970972
SRA SRX5394949

Supplementary file Size Download File type/resource 149.8 Mb (ftp)(http) BW
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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