|
| Status |
Public on Feb 20, 2020 |
| Title |
Limb bud cells Bmp2 |
| Sample type |
RNA |
| |
|
| Source name |
Limb bud cells Bmp2
|
| Organism |
Mus musculus |
| Characteristics |
tissue: limb bud
developmental stage: E12.5 day
strain: ICR
genotype: Bmp2
|
| Treatment protocol |
Limb bud cells were infected with control or Bmp2 adenovirus.
|
| Growth protocol |
Limb bud cells were cultured with α-ΜΕΜ containing 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Nucleospin RNA kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from mouse Lumb bud cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Feb 22, 2019 |
| Last update date |
Feb 20, 2020 |
| Contact name |
Yoshifumi Takahata |
| E-mail(s) |
yo.takahata0331@gmail.com
|
| Phone |
+81-6-6879-2887
|
| Organization name |
Osaka University
|
| Department |
Dentistry
|
| Lab |
Biochemistry
|
| Street address |
1-8
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
5650871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE126945 |
Expression data from Limb bud cells |
|
