|
Status |
Public on Feb 26, 2019 |
Title |
Sample_82540 HNSCC cell line |
Sample type |
SRA |
|
|
Source name |
RNA from Cell Lines
|
Organism |
Homo sapiens |
Characteristics |
sample id: 82540 cell line: HNSCC
|
Treatment protocol |
10% fetal bovine serum, 7µg/mL penicillin/streptomycin and 1% Non-essential amino acids in 5% CO2 incubator
|
Growth protocol |
Cell lines were in log phase growth
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen Allprep Kit Illumina TruSEQ Stranded Total RNA
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Description |
HSC4 FPKM_ProcessedData.xlsx
|
Data processing |
Read quality was assessed for each cohort using FastQC (v0.11.5) Read alignment was performed using STAR (v2.5.3a) Cufflinks (v2.2.1) was used to compute FPKM Genome_build: hg19 Supplementary_files_format_and_content: Excel spreadsheet with FPKM values for each Cell line
|
|
|
Submission date |
Feb 23, 2019 |
Last update date |
Feb 26, 2019 |
Contact name |
Chad Brenner |
Organization name |
University of Michigan
|
Street address |
1500 E Medical Center Drive / Cancer Center 6430
|
City |
Ann Arbor |
State/province |
Michigan |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL20301 |
Series (1) |
GSE126975 |
Transcriptomic profiling of head and neck squamous cell carcinoma cell lines |
|
Relations |
BioSample |
SAMN10992846 |
SRA |
SRX5413979 |