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Sample GSM3619585 Query DataSets for GSM3619585
Status Public on Apr 23, 2019
Title naive Stau2 mice, replicate 1
Sample type RNA
 
Source name naive Stau2 mice_brain tissue
Organism Mus musculus
Characteristics strain background: C57Bl/6J
genotype/variation: B6.129P2-Stau2Gt(RRG396)Byg
age: 16-20 weeks
Sex: male
time point: before behavioural training
tissue: brain
Treatment protocol All behavioral tests were performed under identical environmental conditions. Between individual testing phases, animals were kept in their housing cages. 4 months old male Stau2GT and WT mice were exposed to a stimulation of exploratory and motor behavior for 4 consecutive weeks by different behavioral test (open field, novel object recognition/location, Barnes maze)
Growth protocol Adult male B6.129P2-Stau2Gt(RRG396)Byg mice and WT C57Bl/6J mice (16 weeks to 20 weeks) were housed in groups of 2-5 animals with a 12-h light, 12-h dark light cycle in individually ventilated cages. Mice had free excess to autoclaved water and food.
Extracted molecule total RNA
Extraction protocol Total mRNA was obtained from brain samples using TRIzol (Ambion) according to the manufacturer´s protocol. Genomic DNA was depleted using the Mini RNeasy kit (Qiagen). cDNA was synthesized from purified mRNA by reverse transcription using Superscript III reverse transcriptase (Invitrogen) according to the manufacturer´s manual
Label biotin
Label protocol Standard Affymetrix protocol.
 
Hybridization protocol Standard Affymetrix protocol.
Scan protocol Standard Affymetrix protocol.
Description MJ_03222016_KIE_4_(MoGene-2_0-st)
Data processing R/Bioconductor, rma normalization, default parameters
 
Submission date Feb 25, 2019
Last update date Apr 23, 2019
Contact name Tobias Straub
E-mail(s) tstraub@med.uni-muenchen.de
Organization name LMU Munich
Department Biomedical Center, Bioinformatics
Street address Großhadener Str. 9
City Martinsried
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL16570
Series (1)
GSE126996 Altered Glutamate Receptor Ionotropic Delta Subunit 2 expression in Stau2 deficient cerebellar Purkinje cells in the adult brain

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
17200001 6.75
17200003 6.74
17200005 5.29
17200007 5.86
17200009 5.78
17200011 5.72
17200013 6.07
17200015 6.65
17200017 3.21
17200019 4.82
17200021 6.16
17200023 6.5
17200025 5.76
17200027 6.39
17200029 4.7
17200031 3.96
17200033 5.03
17200035 3.97
17200037 6.4
17200039 3.95

Total number of rows: 41345

Table truncated, full table size 561 Kbytes.




Supplementary file Size Download File type/resource
GSM3619585_MJ_03222016_KIE_4_MoGene-2_0-st_.CEL.gz 9.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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