|
| Status |
Public on Apr 23, 2019 |
| Title |
trained wildtype mice, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
trained wildtype mice_brain tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6J
genotype/variation: wild type
age: 16-20 weeks
Sex: male
time point: after behavioural training
tissue: brain
|
| Treatment protocol |
All behavioral tests were performed under identical environmental conditions. Between individual testing phases, animals were kept in their housing cages. 4 months old male Stau2GT and WT mice were exposed to a stimulation of exploratory and motor behavior for 4 consecutive weeks by different behavioral test (open field, novel object recognition/location, Barnes maze)
|
| Growth protocol |
Adult male B6.129P2-Stau2Gt(RRG396)Byg mice and WT C57Bl/6J mice (16 weeks to 20 weeks) were housed in groups of 2-5 animals with a 12-h light, 12-h dark light cycle in individually ventilated cages. Mice had free excess to autoclaved water and food.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA was obtained from brain samples using TRIzol (Ambion) according to the manufacturer´s protocol. Genomic DNA was depleted using the Mini RNeasy kit (Qiagen). cDNA was synthesized from purified mRNA by reverse transcription using Superscript III reverse transcriptase (Invitrogen) according to the manufacturer´s manual
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
MJ_07222015_KIE_2_(MoGene-2_0-st)
|
| Data processing |
R/Bioconductor, rma normalization, default parameters
|
| |
|
| Submission date |
Feb 25, 2019 |
| Last update date |
Apr 23, 2019 |
| Contact name |
Tobias Straub |
| E-mail(s) |
tstraub@med.uni-muenchen.de
|
| Organization name |
LMU Munich
|
| Department |
Biomedical Center, Bioinformatics
|
| Street address |
Großhadener Str. 9
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE126996 |
Altered Glutamate Receptor Ionotropic Delta Subunit 2 expression in Stau2 deficient cerebellar Purkinje cells in the adult brain |
|
