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Sample GSM3635982 Query DataSets for GSM3635982
Status Public on Oct 04, 2019
Title Granuloma_macrophages_Balb/c_control_2
Sample type RNA
 
Source name Macrophages isolated from poly-acryl amide phagocyte traps
Organism Mus musculus
Characteristics strain: BALB/c
cell type: macrophages
infection status: control
Treatment protocol Macrophages where isolated from the primary phagocyte mixture by adherence overnight and either infected with 5x MOI of metacyclical Leishmania major or left untreated as uninfected controls for 4h a 37°C
Growth protocol Phagocytes where isolated from phagocyte traps according to the protocol published in Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils, and Macrophages by John et al., 2013 in Methods in molecular biology, DOI: 10.1007/978-1-62703-227-8_27
Extracted molecule total RNA
Extraction protocol Total RNA extraction was performed using a Quiagen RNeasy micro kit.
Label Biotin
Label protocol Labeling was done using a 3'IVT kit according to the manufacturers instructions.
 
Hybridization protocol GeneChips were washed and stained in an Affymetrix Fluidics Station 450
Scan protocol Scanning was performed on a GeneChip Scanner 3000 7G
Description Primary murine macrophages isolated from phagocyte traps
Data processing Data was normalised using the MAS5 algorithm in Affymetrix MicroArray Suite
 
Submission date Feb 28, 2019
Last update date Oct 04, 2019
Contact name Niels-Arne Münck
Organization name University of Münster
Department Insitute of Immunology
Street address Roentgenstraße 21
City Münster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL81
Series (1)
GSE127541 Gene Expression by granuloma macrophages from resistant and susceptible mice after 4h of phagocytosis of metacyclical Leishmania major

Data table header descriptions
ID_REF
VALUE Probe signals are given as linear values
ABS_CALL
Detection P-Value

Data table
ID_REF VALUE ABS_CALL Detection P-Value
160069_at 44.3 P 0.000219
96785_at 64.1 P 0.002228
93557_at 215.6 P 0.000388
162204_r_at 35.2 P 0.005859
103404_at 271.1 P 0.001141
92480_f_at 78.6 P 0.000266
160617_at 303 P 0.000959
102854_s_at 42.5 P 0.003585
95617_at 26.7 P 0.000805
94403_at 224.5 P 0.000562
104058_at 418.7 P 0.000219
97485_at 467 P 0.000219
100323_at 245.6 P 0.000322
96836_r_at 16 P 0.000562
103496_at 44.4 P 0.001892
97844_at 178.5 P 0.000219
160894_at 143 P 0.000388
95419_at 130.4 P 0.002228
93697_at 135.8 P 0.001892
96724_r_at 40 P 0.001354

Total number of rows: 12460

Table truncated, full table size 311 Kbytes.




Supplementary file Size Download File type/resource
GSM3635982_BALB_cntrl_2.CEL.gz 2.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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