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Sample GSM3635983 Query DataSets for GSM3635983
Status Public on Oct 04, 2019
Title Granuloma_macrophages_Balb/c_infected_ 2
Sample type RNA
 
Source name Macrophages isolated from poly-acryl amide phagocyte traps
Organism Mus musculus
Characteristics strain: BALB/c
cell type: macrophages
infection status: infected
Treatment protocol Macrophages where isolated from the primary phagocyte mixture by adherence overnight and either infected with 5x MOI of metacyclical Leishmania major or left untreated as uninfected controls for 4h a 37°C
Growth protocol Phagocytes where isolated from phagocyte traps according to the protocol published in Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils, and Macrophages by John et al., 2013 in Methods in molecular biology, DOI: 10.1007/978-1-62703-227-8_27
Extracted molecule total RNA
Extraction protocol Total RNA extraction was performed using a Quiagen RNeasy micro kit.
Label Biotin
Label protocol Labeling was done using a 3'IVT kit according to the manufacturers instructions.
 
Hybridization protocol GeneChips were washed and stained in an Affymetrix Fluidics Station 450
Scan protocol Scanning was performed on a GeneChip Scanner 3000 7G
Description Primary murine macrophages isolated from phagocyte traps
Data processing Data was normalised using the MAS5 algorithm in Affymetrix MicroArray Suite
 
Submission date Feb 28, 2019
Last update date Oct 04, 2019
Contact name Niels-Arne Münck
Organization name University of Münster
Department Insitute of Immunology
Street address Roentgenstraße 21
City Münster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL81
Series (1)
GSE127541 Gene Expression by granuloma macrophages from resistant and susceptible mice after 4h of phagocytosis of metacyclical Leishmania major

Data table header descriptions
ID_REF
VALUE Probe signals are given as linear values
ABS_CALL
Detection P-Value

Data table
ID_REF VALUE ABS_CALL Detection P-Value
160069_at 17.1 P 0.002617
96785_at 18.7 A 0.089405
93557_at 74.7 P 0.000219
162204_r_at 13.9 P 0.021484
103404_at 102.9 P 0.002228
92480_f_at 29.2 P 0.000219
160617_at 182.2 P 0.000805
102854_s_at 17.9 P 0.014937
95617_at 12.6 P 0.000805
94403_at 86.2 P 0.001892
104058_at 225.8 P 0.000219
97485_at 219.1 P 0.000219
100323_at 99.8 P 0.000562
96836_r_at 7.5 P 0.000562
103496_at 21.4 P 0.008689
97844_at 98.4 P 0.000219
160894_at 83 P 0.000673
95419_at 68.4 P 0.008689
93697_at 60.8 P 0.011447
96724_r_at 8.8 P 0.019304

Total number of rows: 12460

Table truncated, full table size 311 Kbytes.




Supplementary file Size Download File type/resource
GSM3635983_BALB_lm_2.CEL.gz 2.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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