|
| Status |
Public on Nov 28, 2019 |
| Title |
D46 |
| Sample type |
RNA |
| |
|
| Source name |
Tonsil
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Large B-cell lymphopma with IRF4 rearrangement
gender: male
tissue: FFPE
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA from tumor cell-rich areas using RNeasy Mini kit protocol.
|
| Label |
Nanostring Reporter Probes
|
| Label protocol |
Samples were labeled with Nanostring Reporter Probes.
|
| |
|
| Hybridization protocol |
hybridization of the samples and Nanostring reporter/capture probes was performed at 65ºC for 16 hours.
|
| Scan protocol |
cartridges were scanned and counted using the Nanostring scanner at its higher resolution (555 FOVs).
|
| Description |
nCounter PanCancer Immune Profiling Panel
|
| Data processing |
Normalitzation with nSolver Analysis Software 4.0. Standard normalization uses a combination of Positive Control Normalization, which uses synthetic positive control targets, and CodeSet Content Normalization, which uses housekeeping genes, to apply a sample-specific correction factor to all the target probes within thatsample lane.
|
| |
|
| Submission date |
Mar 14, 2019 |
| Last update date |
Nov 29, 2019 |
| Contact name |
Joan Enric Ramiz-Zaldivar |
| E-mail(s) |
ramis@clinic.cat
|
| Phone |
+34 932275400
|
| Organization name |
IDIBAPS
|
| Department |
Molecular Pathology Laboratory
|
| Lab |
Centre Esther Koplowitz (CEK)
|
| Street address |
Rosselló, 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL19965 |
| Series (2) |
| GSE128293 |
Large B-Cell Lymphomas in Pediatric and Young Adults Display Clinically Relevant Molecular Features Distinguishable from Adult Counterparts [Nanostring] |
| GSE128294 |
Large B-Cell Lymphomas in Pediatric and Young Adults Display Clinically Relevant Molecular Features Distinguishable from Adult Counterparts |
|
