|
Status |
Public on Nov 28, 2019 |
Title |
D63 |
Sample type |
RNA |
|
|
Source name |
Laringe
|
Organism |
Homo sapiens |
Characteristics |
cell type: Large B-cell lymphopma with IRF4 rearrangement gender: female tissue: FFPE
|
Treatment protocol |
none
|
Growth protocol |
none
|
Extracted molecule |
total RNA |
Extraction protocol |
Extraction of total RNA from tumor cell-rich areas using RNeasy Mini kit protocol.
|
Label |
Nanostring Reporter Probes
|
Label protocol |
Samples were labeled with Nanostring Reporter Probes.
|
|
|
Hybridization protocol |
hybridization of the samples and Nanostring reporter/capture probes was performed at 65ºC for 16 hours.
|
Scan protocol |
cartridges were scanned and counted using the Nanostring scanner at its higher resolution (555 FOVs).
|
Description |
nCounter PanCancer Immune Profiling Panel
|
Data processing |
Normalitzation with nSolver Analysis Software 4.0. Standard normalization uses a combination of Positive Control Normalization, which uses synthetic positive control targets, and CodeSet Content Normalization, which uses housekeeping genes, to apply a sample-specific correction factor to all the target probes within thatsample lane.
|
|
|
Submission date |
Mar 14, 2019 |
Last update date |
Nov 29, 2019 |
Contact name |
Joan Enric Ramiz-Zaldivar |
E-mail(s) |
ramis@clinic.cat
|
Phone |
+34 932275400
|
Organization name |
IDIBAPS
|
Department |
Molecular Pathology Laboratory
|
Lab |
Centre Esther Koplowitz (CEK)
|
Street address |
Rosselló, 153
|
City |
Barcelona |
ZIP/Postal code |
08036 |
Country |
Spain |
|
|
Platform ID |
GPL19965 |
Series (2) |
GSE128293 |
Large B-Cell Lymphomas in Pediatric and Young Adults Display Clinically Relevant Molecular Features Distinguishable from Adult Counterparts [Nanostring] |
GSE128294 |
Large B-Cell Lymphomas in Pediatric and Young Adults Display Clinically Relevant Molecular Features Distinguishable from Adult Counterparts |
|