GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM367233 Query DataSets for GSM367233
Status Public on Apr 01, 2009
Title S.cerevisiae and S.paradoxus at heatshock (35C) repeat #2 Cy5
Sample type RNA
Source name S. cerevisiae (BY4741 ho::kanMX4 MATa), S. paradoxus (CBS432 ho::nat1 MATα)
Organisms Saccharomyces cerevisiae; Saccharomyces paradoxus
Characteristics strain: S. cerevisiae (BY4741), S. paradoxus (CBS432)
Growth protocol heat-shock (YPD at 35°C)
All samples were collected at log-phase growth. Starters were grown over night, dluted to OD(600)=0.1 in 10ml medium and harvested ~5 hours after dilution when OD(600)=~0.5
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using MasterPure™ Yeast RNA purification Kit (EPICENTRE).
Label Cy5
Label protocol Agilent Low RNA Input Amplificarion Kit (LRIAK) plus, two color p/n 5188-5340
Hybridization protocol Agilent Gene Expression Hybridization Kit p/n 5188-5242
Scan protocol Arrays were scanned using Agilent Microarray Scanner (p/n G2565BA) with a 5 micron resolution and photomultiplier tube (PMT) set to 100 and then rescanned with PMT=10. The images analyzed were from the PMT=100 unless data has reached saturation. The raw images were analyzed and quantified using Spotreader software (Niles scientific).
Description Comparative analysis of gene expression in two yeast species and their interspecific hybrid
cells were grown to log-phase at 30° and then transferred to 35° for three hours
Data processing Raw hybridization intensities were background subtracted and log2-transformed. Results of different samples from the same condition were converted into the same distribution using percentile normalization. Probes were then sorted into 10 bins of similar %GC and the log-intensities of each bin were renormalized to the mean and Std of all probes. Finally, the normalized expression was averaged over the technical repeats of each probe (each probe was placed at 3-4 different positions on the array).
Submission date Feb 04, 2009
Last update date Feb 04, 2009
Contact name Itay Tirosh
Organization name Weizmann Institute of Science
Department Molecular genetics
Lab Naama Barkai
Street address herzl
City Rehovot
ZIP/Postal code 76100
Country Israel
Platform ID GPL8157
Series (1)
GSE14708 Comparative analysis of gene expression in two yeast species and their interspecific hybrid

Data table header descriptions
VALUE Normalized log2 of hybridization intensity

Data table
1_1_2678 9.45835
2_1_2678 9.99891
1_1_2836 11.0575
2_1_2836 11.1451
1_1_2587 10.6381
2_1_2587 10.5216
1_1_2077 8.9135
2_1_2077 9.0691
1_2_906 7.9131
2_2_906 6.9429
1_2_1164 6.9766
2_2_1164 6.8325
1_2_1932 7.8711
2_2_1932 7.6526
1_2_1404 7.856
2_2_1404 6.5107
1_6_545 14.4614
2_6_545 13.5568
1_6_375 12.6247
2_6_375 13.3625

Total number of rows: 33648

Table truncated, full table size 609 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap