|
| Status |
Public on Feb 10, 2009 |
| Title |
MeCP2 S80A (Cy3) versus WT (Cy5) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mecp2-/y cortical neurons rescued with mutant MeCP2 (S80A)_replicate 2 (dye swap)
|
| Organism |
Mus musculus |
| Characteristics |
age: Embryonic day 15.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA Input Fluorescuent Linear Amplification Kit
|
| |
|
| Channel 2 |
| Source name |
Mecp2-/y cortical neurons rescued with wild-type MeCP2_replicate 2 (dye swap)
|
| Organism |
Mus musculus |
| Characteristics |
age: Embryonic day 15.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA Input Fluorescuent Linear Amplification Kit
|
| |
|
| |
| Hybridization protocol |
Agilent standard procedures
|
| Scan protocol |
Agilent Scanner (5nm, PMT 100% for both channels)
|
| Description |
MeCP2 S80A (Cy3) versus WT (Cy5)
|
| Data processing |
Agilent Feature Extraction 9.5 (Lowess normalization)
|
| |
|
| Submission date |
Feb 04, 2009 |
| Last update date |
Feb 09, 2009 |
| Contact name |
Yi Sun |
| E-mail(s) |
ysun@mednet.ucla.edu
|
| Phone |
310-825-9506
|
| Organization name |
UCLA
|
| Department |
Molecular and Medical Pharmacology
|
| Street address |
635, Charles E. Young Dr. South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE14715 |
Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function |
|
