|
Status |
Public on Feb 07, 2009 |
Title |
hut78Rpg_1, biological rep 3 |
Sample type |
RNA |
|
|
Source name |
Hut 78 cells transduced with a control vector
|
Organism |
Homo sapiens |
Characteristics |
A transformed leukemic cell line
|
Growth protocol |
Hut 78 cells (transduced and untransduced) were cultured in RPMI 1640 Medium with 10% fetal bovine serum, 100 U/mL penicillin, 0.1 mg/mL streptomycin, and 1 x 10-4 M β-mercaptoethanol.
|
Extracted molecule |
total RNA |
Extraction protocol |
The Absolutely RNA® Miniprep Kit (Stratagene, La Jolla, CA) was used to isolate and purify total RNA using the procedure described for tissue culture cells grown in suspension.
|
Label |
biotin
|
Label protocol |
Affymetrix standard procedure
|
|
|
Hybridization protocol |
Affymetrix standard procedure
|
Scan protocol |
Affymetrix standard procedure
|
Description |
A human cell line derived from blood of a patient with Sezary syndrome
|
Data processing |
The data was normalized with Robust Multichip Analysis (RMA)
|
|
|
Submission date |
Feb 06, 2009 |
Last update date |
Aug 28, 2018 |
Contact name |
BCGSC BC Cancer Agency |
E-mail(s) |
support@mouseatlas.org
|
Organization name |
Canada's Michael Smith Genome Sciences Centre
|
Department |
Gene Expression
|
Lab |
Marco Marra
|
Street address |
675 West 10th Avenue
|
City |
Vancouver |
State/province |
BC |
ZIP/Postal code |
V5Z 1L3 |
Country |
Canada |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE14746 |
Identification of HCK and BIN1 as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells |
|
Relations |
Reanalyzed by |
GSE119087 |