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Sample GSM368399 Query DataSets for GSM368399
Status Public on Feb 07, 2009
Title hut78Rpg_1, biological rep 3
Sample type RNA
 
Source name Hut 78 cells transduced with a control vector
Organism Homo sapiens
Characteristics A transformed leukemic cell line
Growth protocol Hut 78 cells (transduced and untransduced) were cultured in RPMI 1640 Medium with 10% fetal bovine serum, 100 U/mL penicillin, 0.1 mg/mL streptomycin, and 1 x 10-4 M β-mercaptoethanol.
Extracted molecule total RNA
Extraction protocol The Absolutely RNA® Miniprep Kit (Stratagene, La Jolla, CA) was used to isolate and purify total RNA using the procedure described for tissue culture cells grown in suspension.
Label biotin
Label protocol Affymetrix standard procedure
 
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description A human cell line derived from blood of a patient with Sezary syndrome
Data processing The data was normalized with Robust Multichip Analysis (RMA)
 
Submission date Feb 06, 2009
Last update date Aug 28, 2018
Contact name BCGSC BC Cancer Agency
E-mail(s) support@mouseatlas.org
Organization name Canada's Michael Smith Genome Sciences Centre
Department Gene Expression
Lab Marco Marra
Street address 675 West 10th Avenue
City Vancouver
State/province BC
ZIP/Postal code V5Z 1L3
Country Canada
 
Platform ID GPL570
Series (1)
GSE14746 Identification of HCK and BIN1 as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE RMA signal intensity (in log base 2)

Data table
ID_REF VALUE
1007_s_at 4.094570685
1053_at 9.283032963
117_at 4.335548766
121_at 6.879998495
1255_g_at 2.436589776
1294_at 6.488564808
1316_at 3.7271436
1320_at 3.621376743
1405_i_at 11.51485943
1431_at 2.868054745
1438_at 4.187589746
1487_at 6.395173954
1494_f_at 3.456759625
1552256_a_at 5.823565031
1552257_a_at 7.562144592
1552258_at 2.839645969
1552261_at 4.321708458
1552263_at 6.092410741
1552264_a_at 8.502205596
1552266_at 2.521398984

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM368399.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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