|
| Status |
Public on Feb 07, 2009 |
| Title |
hut78Sh4Bulk_1, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
Hut 78 cells transduced with AHI-1/sh4 vector for suppression of AHI-1 expression, a bulk population
|
| Organism |
Homo sapiens |
| Characteristics |
The transformed phenotypes can be normalized by suppression of AHI-1
|
| Growth protocol |
Hut 78 cells (transduced and untransduced) were cultured in RPMI 1640 Medium with 10% fetal bovine serum, 100 U/mL penicillin, 0.1 mg/mL streptomycin, and 1 x 10-4 M β-mercaptoethanol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The Absolutely RNA® Miniprep Kit (Stratagene, La Jolla, CA) was used to isolate and purify total RNA using the procedure described for tissue culture cells grown in suspension.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard procedure
|
| |
|
| Hybridization protocol |
Affymetrix standard procedure
|
| Scan protocol |
Affymetrix standard procedure
|
| Description |
A human cell line derived from blood of a patient with Sezary syndrome
|
| Data processing |
The data was normalized with Robust Multichip Analysis (RMA)
|
| |
|
| Submission date |
Feb 06, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
BCGSC BC Cancer Agency |
| E-mail(s) |
support@mouseatlas.org
|
| Organization name |
Canada's Michael Smith Genome Sciences Centre
|
| Department |
Gene Expression
|
| Lab |
Marco Marra
|
| Street address |
675 West 10th Avenue
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 1L3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE14746 |
Identification of HCK and BIN1 as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells |
|
| Relations |
| Reanalyzed by |
GSE119087 |
