|
| Status |
Public on Feb 10, 2009 |
| Title |
control mammary gland, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
control mammary gland
|
| Organism |
Mus musculus |
| Characteristics |
control mammary gland
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted by homogenizing tumors and tissues in 3 ml Trizol reagent (Invitrogen). After phase separation, an equal volume of 70% ethanol was added to the aqueous phase and purified through PureLink Micro-to-Midi Total RNA Purification System (Invitrogen), following manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Model 3000 7G scanner with autoloader
|
| Description |
Gene expression data from control mammary gland.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Feb 09, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Dmitriy Sonkin |
| Organization name |
Novartis Institutes for BioMedical Research
|
| Street address |
250 Massachusetts Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE14753 |
Mammary tumors from K14-cre; ApcCKO/+ mice vs control mammary glands |
|
| Relations |
| Reanalyzed by |
GSE119085 |
