|
| Status |
Public on Apr 01, 2020 |
| Title |
uterine tissue_PKRASG12V_7601 |
| Sample type |
SRA |
| |
|
| Source name |
uterine tissue
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: Ahmr-Cre Pten null
strain: mixed C57BL/6 x 129
genotype: KRAS-G12V
age: 36 weeks
tissue: uterine tissue
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted from mouse uterine tissues that have been stored in RNAlater solution using Qiagen RNeasy Mini Kit.
Sequencing libraries were prepared using KAPA Stranded mRNA seq Kit and run in a single lane using Illumina HiSeq 4000 instrument.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were processed with RNA-seq workflow in CLC Genomics Workbench version 11. Reads were mapped to mouse reference genome GRCm38 with default setting.
Gene expression is estimated with the EM estimation algorithm and reported as TPM (Transcripts Per Million)
Genome_build: GRCm38
Supplementary_files_format_and_content: TPMs
|
| |
|
| Submission date |
Apr 09, 2019 |
| Last update date |
Sep 22, 2020 |
| Contact name |
Kwong-Kwok Wong |
| E-mail(s) |
kkwong@mdanderson.org
|
| Phone |
713-792-0229
|
| Organization name |
UT M. D. Anderson Cancer Center
|
| Department |
Gynecologic Oncology and Reproductive Medicine
|
| Street address |
T4-3900, 1515 Holcombe Boulevard
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE129520 |
Differences in gynecologic tumor development in Amhr2-Cre mice with KRASG12D or KRASG12V mutations |
|
| Relations |
| BioSample |
SAMN11371899 |
| SRA |
SRX5656267 |
