|Public on Feb 04, 2021
|Fasted Knockout 2
|Mouse intestines without PC synthesis in the fasted state
age: 10-12 weeks
|The mice were placed on a high fat diet for 4 days before being fasted overnight. Intestines were excised and opened longitudinally. 3 cm was removed from the geometric middle of the small intetsine (jejunum) and stored in liquid nitrogen.
|Pcyt1aLoxp/LoxP;VilcreERT2 mice were treated with tamoxifen for 5 days to induce Cre recombinase, and LoxP/LoxP control mice were also treated with tamoxifen.
|Trizol extraction folllowed by RNeasy column clean-up.
|Biotin labeled cRNA were prepared according to the standard Affymetrix WT Plus Kit Protocol from 400ng total RNA.
|16-18 hours at 45 degrees Celcius as descibed in Affymetrix protocol.
|Affymetrix GeneChip Scanner 3000
|Gene expression data from mouse intestines without PC synthesis in the fasted state
|Expression Console 126.96.36.199 using RMA normalization; TAC 3
|Apr 14, 2019
|Last update date
|Feb 04, 2021
|René L Jacobs
|University of Alberta
|Agriculture, Food and Nutritional Science; Biochemistry
|Li Ka Shing Centre for Health Research
|Expression data from intestine samples of mice lacking intestinal Pcyt1a, which encodes an enzyme that reguates phosphatidylcholine synthesis.