|
| Status |
Public on Feb 04, 2021 |
| Title |
Fasted Knockout 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse intestines without PC synthesis in the fasted state
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Intestine
age: 10-12 weeks
Sex: female
genotype: Pcyt1aLoxP/LoxP;VilCreERT2
|
| Treatment protocol |
The mice were placed on a high fat diet for 4 days before being fasted overnight. Intestines were excised and opened longitudinally. 3 cm was removed from the geometric middle of the small intetsine (jejunum) and stored in liquid nitrogen.
|
| Growth protocol |
Pcyt1aLoxp/LoxP;VilcreERT2 mice were treated with tamoxifen for 5 days to induce Cre recombinase, and LoxP/LoxP control mice were also treated with tamoxifen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction folllowed by RNeasy column clean-up.
|
| Label |
Biotin
|
| Label protocol |
Biotin labeled cRNA were prepared according to the standard Affymetrix WT Plus Kit Protocol from 400ng total RNA.
|
| |
|
| Hybridization protocol |
16-18 hours at 45 degrees Celcius as descibed in Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from mouse intestines without PC synthesis in the fasted state
|
| Data processing |
Expression Console 1.4.1.46 using RMA normalization; TAC 3
|
| |
|
| Submission date |
Apr 14, 2019 |
| Last update date |
Feb 04, 2021 |
| Contact name |
René L Jacobs |
| E-mail(s) |
rjacobs@ualberta.ca
|
| Organization name |
University of Alberta
|
| Department |
Agriculture, Food and Nutritional Science; Biochemistry
|
| Lab |
Jacobs Lab
|
| Street address |
Li Ka Shing Centre for Health Research
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G2E1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE129776 |
Expression data from intestine samples of mice lacking intestinal Pcyt1a, which encodes an enzyme that reguates phosphatidylcholine synthesis. |
|
