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Sample GSM3721931 Query DataSets for GSM3721931
Status Public on Feb 04, 2021
Title Fasted Control 6
Sample type RNA
 
Source name Mouse intestines without PC synthesis in the fasted state
Organism Mus musculus
Characteristics tissue: Intestine
age: 10-12 weeks
Sex: female
genotype: Pcyt1aLoxP/LoxP
Treatment protocol The mice were placed on a high fat diet for 4 days before being fasted overnight. Intestines were excised and opened longitudinally. 3 cm was removed from the geometric middle of the small intetsine (jejunum) and stored in liquid nitrogen.
Growth protocol Pcyt1aLoxp/LoxP;VilcreERT2 mice were treated with tamoxifen for 5 days to induce Cre recombinase, and LoxP/LoxP control mice were also treated with tamoxifen.
Extracted molecule total RNA
Extraction protocol Trizol extraction folllowed by RNeasy column clean-up.
Label Biotin
Label protocol Biotin labeled cRNA were prepared according to the standard Affymetrix WT Plus Kit Protocol from 400ng total RNA.
 
Hybridization protocol 16-18 hours at 45 degrees Celcius as descibed in Affymetrix protocol.
Scan protocol Affymetrix GeneChip Scanner 3000
Description Gene expression data from mouse intestines without PC synthesis in the fasted state
Data processing Expression Console 1.4.1.46 using RMA normalization; TAC 3
 
Submission date Apr 14, 2019
Last update date Feb 04, 2021
Contact name René L Jacobs
E-mail(s) rjacobs@ualberta.ca
Organization name University of Alberta
Department Agriculture, Food and Nutritional Science; Biochemistry
Lab Jacobs Lab
Street address Li Ka Shing Centre for Health Research
City Edmonton
State/province Alberta
ZIP/Postal code T6G2E1
Country Canada
 
Platform ID GPL16570
Series (1)
GSE129776 Expression data from intestine samples of mice lacking intestinal Pcyt1a, which encodes an enzyme that reguates phosphatidylcholine synthesis.

Data table header descriptions
ID_REF
VALUE Expression Console RMA, Linear signal

Data table
ID_REF VALUE
17459769 3769.088617
17324803 749.6118763
17477979 975.50131
17258168 968.7630302
17439388 304.4370214
17465170 1795.288517
17301452 116.1624519
17472581 1379.567183
17364392 306.5545484
17236620 157.5864849
17520353 232.3249038
17359262 396.1766383
17489363 27.28431654
17505938 64.4451552
17359287 230.7201184
17547624 278.2041248
17329423 1360.574274
17217158 17.1483754
17346528 87.42657643
17426563 14.2214829

Total number of rows: 34472

Table truncated, full table size 699 Kbytes.




Supplementary file Size Download File type/resource
GSM3721931_FastedControl6.CEL.gz 8.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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