|
| Status |
Public on Jun 02, 2009 |
| Title |
A155_7443_early_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Quadriceps femoris
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
tissue: muscle
strain: C57Bl/6 HT transgenic
age at sacrifice (days): 35
treatment: doxycycline stopped age 21 days
|
| Treatment protocol |
early mice were removed from doxycycline at 21 days, and sacrificed at 35 days of age, after 2 weeks of transgene expression. late mice were maintained on doxycycline until 35 days and sacrificed at 49-50 days of age. Single transgenic littermate controls were age, sex-matched,maintained on doxycycline and sacrificed as the early group
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 100mg of quadriceps muscle using RNAB (Campro Scientific, Berlin germany).
|
| Label |
biotin
|
| Label protocol |
RNA was coverted to cDNA (Roche) using an oligod(T) primer and converted to cRNA using In Vitro Triscription labelling kit (Affymetrix
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Mouse Genome 430 2.0 Array. GeneChips were washed and stained on the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000.
|
| Description |
Female C57Bl/6 HT transgenic,doxycyline stopped age 21 days , muscle tissue, age at sacrifice 35 days
|
| Data processing |
Data was normalised using GC-RMA
|
| |
|
| Submission date |
Feb 25, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Lucy R Wedderburn |
| Organization name |
Institute of Child Health, UCL
|
| Department |
Rheumatology
|
| Street address |
30 Guilford Street
|
| City |
London |
| ZIP/Postal code |
WC1N 1EH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE14997 |
Expression data from young and adult mice after over expression of self MHC class l protein in skeletal muscle. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
