NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM374618 Query DataSets for GSM374618
Status Public on Jun 02, 2009
Title A155_7444_late_rep3
Sample type RNA
 
Source name Quadriceps femoris
Organism Mus musculus
Characteristics gender: Female
tissue: muscle
strain: C57Bl/6 HT transgenic
age at sacrifice (days): 50
treatment: doxycycline stopped age 35 days
Treatment protocol early mice were removed from doxycycline at 21 days, and sacrificed at 35 days of age, after 2 weeks of transgene expression. late mice were maintained on doxycycline until 35 days and sacrificed at 49-50 days of age. Single transgenic littermate controls were age, sex-matched,maintained on doxycycline and sacrificed as the early group
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from 100mg of quadriceps muscle using RNAB (Campro Scientific, Berlin germany).
Label biotin
Label protocol RNA was coverted to cDNA (Roche) using an oligod(T) primer and converted to cRNA using In Vitro Triscription labelling kit (Affymetrix
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the Mouse Genome 430 2.0 Array. GeneChips were washed and stained on the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Scanner 3000.
Description Female C57Bl/6 HT transgenic,doxycyline stopped age 35 days , muscle tissue, age at sacrifice 50 days
Data processing Data was normalised using GC-RMA
 
Submission date Feb 25, 2009
Last update date Aug 28, 2018
Contact name Lucy R Wedderburn
Organization name Institute of Child Health, UCL
Department Rheumatology
Street address 30 Guilford Street
City London
ZIP/Postal code WC1N 1EH
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE14997 Expression data from young and adult mice after over expression of self MHC class l protein in skeletal muscle.
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GC-RMA log2 intensity

Data table
ID_REF VALUE
1415670_at 9.427170821
1415671_at 10.62436162
1415672_at 11.49770658
1415673_at 8.528448836
1415674_a_at 9.129581837
1415675_at 7.064327245
1415676_a_at 12.24265895
1415677_at 9.446827587
1415678_at 12.00924037
1415679_at 11.49942182
1415680_at 8.094052577
1415681_at 10.17946724
1415682_at 8.513216063
1415683_at 10.6875348
1415684_at 9.422580997
1415685_at 9.651923279
1415686_at 10.67397165
1415687_a_at 12.70032126
1415688_at 12.23253141
1415689_s_at 8.112169383

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM374618.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap