|
| Status |
Public on Sep 12, 2019 |
| Title |
ecig day 4.5, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse, day4.5 pseudopregnant
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: uterus
time: day 4.5 pseudopregnancy
genotype: wildtype
treatment: ecig
|
| Treatment protocol |
Mice were exposed to E-Cigarette aerosol in a SCIREQ inExpose whole-body inhalation system (Montréal, QC, Canada) for 3 hours/day with 2 puffs/minute. Puff duration was approximately 2 seconds. Vapor was generated using a Joyetech eVic VTC Mini with a 0.15 Ω adjustable-temperature atomizer set to 245° C. The propylene glycol and vegetable glycerin mixture was made fresh in a 55:45 ratio with 24mg/mL nicotine. No flavorings or other adulterants were added. Sham mice were exposed to room air. C57BL/6J females were primed with E-Cigarette for 5 days a week, for 4 weeks before mating to vasectomized males. The presence of a copulatory plug was recorded as day 0.5 and pseudopregnant mice were exposed for each day of pregnancy until euthanasia on the morning of day 4.5
|
| Growth protocol |
C57BL/6J mice were cared for according to the Institution of Animal Care and Use Committee at the University of North Carolina, with 12 hour light cycles and normal rodent chow.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Trizol with two aqueous phase separations using chloroform and 1-Bromo-3-chloropropane, precipitated with ethanol, and cleaned up and eluted with Qiagen RNEasy mini prep kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to Affymetrix protocol.
|
| |
|
| Hybridization protocol |
GeneChips were washed and stained in the 2 GeneChip Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G Plus with Autoloader
|
| Description |
gene expression data from day 4.5 pseudopregnant uterus, e-cigarette exposed
ecig_1
|
| Data processing |
Data was analyzed using Partek Genomics Suite with Gene Expression Workflow followed by quantile normalization
|
| |
|
| Submission date |
May 13, 2019 |
| Last update date |
Sep 12, 2019 |
| Contact name |
Kathleen M Caron |
| E-mail(s) |
kathleen_caron@med.unc.edu
|
| Organization name |
University of North Carolin
|
| Department |
Cell Biology and Physiology
|
| Street address |
111 Mason Farm Road, CB7545
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE131077 |
Gene Expression Changes from E-Cigarette Exposure During Early Pregnancy |
|
