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Sample GSM3781187 Query DataSets for GSM3781187
Status Public on Mar 11, 2021
Title NC4
Sample type RNA
 
Source name Plasma
Organism Homo sapiens
Characteristics tissue: whole blood
gender: male
age: 63y
disease state: Healthy volunteers
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label Cy5
Label protocol cDNA labeled with a fluorescent dye (Cy5-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
 
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description circRNA
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
 
Submission date May 20, 2019
Last update date Mar 11, 2021
Contact name Zijuan Wu
E-mail(s) xywuzj@163.com
Phone 2583781120
Organization name The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital
Street address 300 Guangzhou Road, Nanjing, 210029, China
City Nanjing
State/province Jiangsu
ZIP/Postal code 210029
Country China
 
Platform ID GPL26692
Series (1)
GSE131469 CLL plasma circRNA microarray

Data table header descriptions
ID_REF
VALUE log2 value of 75 percentile normalized value.

Data table
ID_REF VALUE
hsa_circ_0134491_CBC1 6.651424436
hsa_circ_0109320_CBC1 7.115350289
hsa_circ_0037542_CBC1 7.896172963
hsa_circ_0076045_CBC1 6.671886212
hsa_circ_0000583_CBC1 4.627173079
hsa_circ_0051890_CBC1 7.808219833
hsa_circ_0076048_CBC1 7.178014512
hsa_circ_0081140_CBC1 4.627173079
hsa_circ_0089761_CBC1 4.627173079
hsa_circ_0051903_CBC1 8.263345039
hsa_circ_0051901_CBC1 5.928801365
hsa_circ_0121207_CBC1 5.797984319
hsa_circ_0110603_CBC1 5.907316763
hsa_circ_0089763_CBC1 6.042301291
hsa_circ_0089762_CBC1 6.406175011
hsa_circ_0123961_CBC1 5.783799736
hsa_circ_0076992_CBC1 6.799849614
hsa_circ_0069472_CBC1 5.985621469
hsa_circ_0027098_CBC1 7.072348768
hsa_circ_0009748_CBC1 5.952230569

Total number of rows: 3656

Table truncated, full table size 121 Kbytes.




Supplementary file Size Download File type/resource
GSM3781187_US10313827_258421710135_S01_GE1_107_Sep09_red_only_2_1_4.txt.gz 6.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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