|
| Status |
Public on Mar 27, 2009 |
| Title |
H13B ESC (set of 12) |
| Sample type |
RNA |
| |
|
| Source name |
Human Embryonic Stem Cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: H13B passage 43 Human Embryonic Stem Cells
|
| Treatment protocol |
Lentiviral transduction of fibroblast cells was performed as previously described.
|
| Growth protocol |
iPS cells were maintained on irradiated mouse embryonic fibroblasts (MEF) as previously described. Fibroblast cells (GM03813 and GM03814, Coriell Inst.) were cultured in Minimum Essential Medium (Eagle) (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (HyClone Laboratories).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion or Tri-Reagent (Sigma).
|
| Label |
biotin
|
| Label protocol |
Approximately 3 µg of RNA from each sample was labeled using the MessageAmp™ Biotin II-Enhanced IVT kit (Ambion) following manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
All arrays were hybridized at 45ºC for 16 hours and scanned using an AFX GC3000 G7 scanner.
|
| Scan protocol |
AFX GC3000 G7 scanner
|
| Description |
Human Embryonic Stem Cell line
|
| Data processing |
RMA
|
| |
|
| Submission date |
Mar 10, 2009 |
| Last update date |
Jan 18, 2011 |
| Contact name |
Victor Ruotti |
| E-mail(s) |
ruotti@wisc.edu
|
| Phone |
608-890-0182
|
| Fax |
608-890-0167
|
| Organization name |
UW Madison
|
| Department |
Biotechnology
|
| Lab |
Thomson's Lab
|
| Street address |
425 Henry Mall
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE15148 |
Human induced pluripotent stem cells free of exogenous DNA are derived with episomal vectors |
| GSE15176 |
Human induced pluripotent stem cells free of exogenous DNA are derived with episomal vectors (fig 4.a) |
|
| Relations |
| Reanalyzed by |
GSM500988 |
| Reanalyzed by |
GSM656448 |
