|
| Status |
Public on Mar 14, 2009 |
| Title |
bcl6 in germinal center cells replicate2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
genomic DNA (input)
|
| Organism |
Homo sapiens |
| Characteristics |
type: Input
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
sonicated genomic DNA, then purify by qiagen column.
|
| Label |
Cy3
|
| Label protocol |
described in www.nimblegen.com
|
| |
|
| Channel 2 |
| Source name |
chromatinIP products which are genomic regions bound by BCL6
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: germinal center cells
type: ChIP
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
chromatinIP products which are genomic regions bound by BCL6, then purify by qiagen column.
|
| Label |
Cy5
|
| Label protocol |
described in www.nimblegen.com
|
| |
|
| |
| Hybridization protocol |
described in www.nimblegen.com
|
| Scan protocol |
described in www.nimblegen.com
|
| Description |
chip-on-chip is performing in germinal center cells by BCL6 antibody.
|
| Data processing |
Take all the probes in the promoter region and do Log2 transformed IP/INPUT(Cy5/Cy3). Then use the maximal summary of three connected probes as the bound intensity of the promoter.
|
| |
|
| Submission date |
Mar 10, 2009 |
| Last update date |
Mar 18, 2009 |
| Contact name |
weimin ci |
| E-mail(s) |
wec2007@med.cornell.edu
|
| Phone |
12127467622
|
| Organization name |
weill cornell college of medicine
|
| Department |
medicine
|
| Lab |
ari melnick
|
| Street address |
1300 York ave
|
| City |
new york |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL8275 |
| Series (1) |
|
