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Sample GSM379717 Query DataSets for GSM379717
Status Public on Jun 30, 2011
Title Patient6_339_GH_Day1_GH0_2PM (4 days)
Sample type RNA
 
Source name Milk Fat Globule
Organism Homo sapiens
Characteristics sample: Milk Fat Globule
Extracted molecule total RNA
Extraction protocol RNA was isolated from the milk fat globule using Trizol following the manufacturer's protocol
Label Biotin
Label protocol 440 ng total RNA was used for in vitro transcription, overnight for 14h using biotin-11-dUTP for labeling of the cRNA product using the Illumina®TotalPrep RNA amplification kit (Ambion, Inc., Austin, TX)
 
Hybridization protocol 750 ng of labeled cRNAs were hybridized to the Human Ref 8 V2 Beadchip arrays (Illumina Inc., San Diego, CA) at 55oC overnight (16h) following the Illumina Whole-Genome Gene Expression Protocol for BeadStation (Doc. # 111226048 Rev. B, Illumina Inc) and stained with 1 ug/ml streptavidin-Cy3 (Amersham Biosciences, Piscataway, NJ) for visualization
Scan protocol Probe arrays were scanned using the BeadArray reader (Factor 1)
Description Day1_GH0_2PM
Data processing Gene expression analysis was performed using the Sentrix BeadChip and BeadStation system from Illumina, Inc
 
Submission date Mar 12, 2009
Last update date Jun 30, 2011
Contact name Patricia Maningat
E-mail(s) mmaningat@mail.rockefeller.edu
Organization name Baylor College of Medicine
Department CNRC
Street address 1100 Bates St
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platform ID GPL6104
Series (1)
GSE15208 Gene Expression in the Human Mammary Epithelium during Lactation: the Milk Fat Globule Transcriptome through 4 Days

Data table header descriptions
ID_REF
VALUE cubic spline normalized signal intensity
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_1809034 120.6219 47 4.437916 0.005649718
ILMN_1660305 299.7883 34 11.60865 0
ILMN_1792173 190.6428 36 8.274604 0.001412429
ILMN_1762337 61.29906 47 2.147255 0.6483051
ILMN_1736007 62.94156 54 2.083235 0.5748588
ILMN_1787689 67.4418 58 2.000011 0.3460452
ILMN_1731507 53.24543 37 2.816609 0.9745763
ILMN_1814316 75.0659 42 2.785917 0.1370057
ILMN_1745607 57.49602 48 2.276001 0.8545198
ILMN_1757454 62.22762 44 2.988066 0.6059322
ILMN_1735045 78.51106 34 3.465584 0.09180791
ILMN_1680754 73.7525 45 3.622123 0.1567797
ILMN_1659452 59.18442 36 2.807406 0.7627119
ILMN_1767388 132.3329 37 6.349831 0.005649718
ILMN_1673870 72.86208 37 2.899518 0.1779661
ILMN_1675204 62.20411 55 2.479842 0.6059322
ILMN_1755321 150.0727 48 4.951807 0.002824859
ILMN_1698554 1305.672 50 34.26637 0
ILMN_1805938 56.72887 51 2.051337 0.8884181
ILMN_1676336 79.19987 48 2.625113 0.08192091

Total number of rows: 22184

Table truncated, full table size 907 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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