|
| Status |
Public on Mar 15, 2010 |
| Title |
DU145 - 21days |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
prostate cancer cell line DU145 - exposed to RB - 21 days
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DU145
gender: male
|
| Treatment protocol |
Cells were passaged several times in log phase cultures. Experimental dishes were sandwiched with RBs. Another sets of dishes as reference samples were cultured without RBs.
|
| Growth protocol |
The DU145, PC-3 and LNCaP cell lines were obtained from American Type Culture Collection (Manassas, VA, USA). DU145 cells were maintained in minimum essential medium supplemented with 10% heat-inactivated fetal calf serum, 2 mM L-glutamine, 100 U/ml penicillin G and 0.1 mM non-essential amino acids in an atmosphere of 5% CO2 at 37°C. PC-3 and LNCaP cells were maintained in F-12K medium and RPMI medium with the same supplements, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol (Invitrogen, CA) and purified using RNeasy kit (QIAGEN, Hilden, Germany) following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
4 µg of total RNA were labeled with Cy3 or Cy5 monoreactive dyes (Amersham, Buckinghamshire, UK) using RNA Transcript SureLABEL™ Core Kit (TaKaRa Bio) according to the manufacture’s instructions
|
| |
|
| Channel 2 |
| Source name |
prostate cancer cell line DU145 - control - 21 days
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DU145
gender: male
|
| Treatment protocol |
Cells were passaged several times in log phase cultures. Experimental dishes were sandwiched with RBs. Another sets of dishes as reference samples were cultured without RBs.
|
| Growth protocol |
The DU145, PC-3 and LNCaP cell lines were obtained from American Type Culture Collection (Manassas, VA, USA). DU145 cells were maintained in minimum essential medium supplemented with 10% heat-inactivated fetal calf serum, 2 mM L-glutamine, 100 U/ml penicillin G and 0.1 mM non-essential amino acids in an atmosphere of 5% CO2 at 37°C. PC-3 and LNCaP cells were maintained in F-12K medium and RPMI medium with the same supplements, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol (Invitrogen, CA) and purified using RNeasy kit (QIAGEN, Hilden, Germany) following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
4 µg of total RNA were labeled with Cy3 or Cy5 monoreactive dyes (Amersham, Buckinghamshire, UK) using RNA Transcript SureLABEL™ Core Kit (TaKaRa Bio) according to the manufacture’s instructions
|
| |
|
| |
| Hybridization protocol |
hybridization buffer : 50% formamide-6 x SSC-0.2% SDS-5 x denhardt’s-0.2 mg/ml denatured salmon sperm, hybridization : at 70°C for 16 h, washing : three times with 2× SSC, 0.2% SDS at 65°C for 10 min, and rinsed once with 0.05 × SSC at room temperature
|
| Scan protocol |
Scanned on an GMS 428 arrayer (Affymetrix Inc)
Images were quantified using ImaGene version 6.0 (Biodiscovery Inc., CA, USA)
|
| Description |
DU145 21days
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Data analysis was performed using the microarray data analysis software, Expressionist (GeneData AG, Basel, Switzerland).
|
| |
|
| Submission date |
Mar 17, 2009 |
| Last update date |
Mar 15, 2010 |
| Contact name |
Hiroki Shima |
| E-mail(s) |
shima@siqm.org
|
| Phone |
81-6-6372-8755
|
| Organization name |
SHIMA Institution for Quantum Medicine
|
| Street address |
3-7-41 Nakatsu YAMAMOTO Bild. 4F. Kitaku
|
| City |
Osaka |
| ZIP/Postal code |
531-0071 |
| Country |
Japan |
| |
|
| Platform ID |
GPL8312 |
| Series (2) |
| GSE15260 |
Effects of far-infrared rays on 3 human prostate cancer cell lines |
| GSE15266 |
Effects of far-infrared rays on prostate cancer cells and normal prostate epithelial cells |
|
