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Sample GSM38159 Query DataSets for GSM38159
Status Public on Feb 08, 2005
Title MGUS-019
Sample type RNA
 
Source name CD138+ plasma cells obtained from bone marrow of MGUS patient
Organism Homo sapiens
Extracted molecule total RNA
 
Description gene expression profiles of purified plasma cells (PCs) obtained from a monoclonal gammopathy of undetermined significance (MGUS). PCs were purified from bone marrow samples, after red blood cell lysis with 0.86% ammonium chloride, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (90%) was assessed by morphology and flow cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values. Arrays normalization was performed normalizing the signals to the target intensity of TGT=100.
Keywords = Gene expression profiling
Keywords = IGH translocations
Keywords = MGUS
Keywords = Multiple Myeloma
Keywords = Plasma Cell Leukemia
 
Submission date Dec 27, 2004
Last update date May 27, 2005
Contact name Luca Agnelli
E-mail(s) luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
Phone +390223903581
Organization name IRCCS Istituto Nazionale dei Tumori
Department Department of Advanced Diagnostics
Street address Venezian 1
City MILAN
ZIP/Postal code 20133
Country Italy
 
Platform ID GPL96
Series (1)
GSE2113 Plasma cell dyscrasias expression profiles associated with distinct IGH translocations in multiple myeloma

Data table header descriptions
ID_REF Affymetrix probes number ID
VALUE Average difference intensity (See Affymetrix Literature)
ABS_CALL The call in the absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)
DETECTION P-VALUE the detection p-value in the absolute analysis used to assign the call present (P), absent (A) or marginal (M)

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 134.2 P 0.006187
AFFX-BioB-M_at 164.3 P 0.000662
AFFX-BioB-3_at 72.1 P 0.021902
AFFX-BioC-5_at 330.2 P 0.000225
AFFX-BioC-3_at 182.9 P 0.000662
AFFX-BioDn-5_at 344.7 P 0.000972
AFFX-BioDn-3_at 1508.1 P 0.000225
AFFX-CreX-5_at 2905 P 0.000044
AFFX-CreX-3_at 6064.1 P 0.000044
AFFX-DapX-5_at 19.5 A 0.108979
AFFX-DapX-M_at 11.7 A 0.287743
AFFX-DapX-3_at 3.3 A 0.916408
AFFX-LysX-5_at 1.9 A 0.749204
AFFX-LysX-M_at 3 A 0.883887
AFFX-LysX-3_at 1.7 A 0.804734
AFFX-PheX-5_at 1.9 A 0.843268
AFFX-PheX-M_at 3.9 A 0.659339
AFFX-PheX-3_at 8.2 A 0.659339
AFFX-ThrX-5_at 4.4 A 0.794268
AFFX-ThrX-M_at 13.9 A 0.48511

Total number of rows: 22283

Table truncated, full table size 579 Kbytes.




Supplementary file Size Download File type/resource
GSM38159.CEL.gz 3.4 Mb (ftp)(http) CEL

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