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Sample GSM38169 Query DataSets for GSM38169
Status Public on Feb 08, 2005
Title MM-026
Sample type RNA
 
Source name CD138+ plasma cells obtained from bone marrow of MM patient
Organism Homo sapiens
Extracted molecule total RNA
 
Description gene expression profiles of purified plasma cells (PCs) obtained from a multiple myeloma patient (MM). PCs were purified from bone marrow samples, after red blood cell lysis with 0.86% ammonium chloride, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs (94%) was assessed by morphology and flow cytometry. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A chip following the manufacturer's instructions. After scanning, the image was processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values. Arrays normalization was performed normalizing the signals to the target intensity of TGT=100.
Keywords = Gene expression profiling
Keywords = IGH translocations
Keywords = MGUS
Keywords = Multiple Myeloma
Keywords = Plasma Cell Leukemia
 
Submission date Dec 27, 2004
Last update date May 27, 2005
Contact name Luca Agnelli
E-mail(s) luca.agnelli@istitutotumori.mi.it, luca.agnelli@gmail.com
Phone +390223903581
Organization name IRCCS Istituto Nazionale dei Tumori
Department Department of Advanced Diagnostics
Street address Venezian 1
City MILAN
ZIP/Postal code 20133
Country Italy
 
Platform ID GPL96
Series (1)
GSE2113 Plasma cell dyscrasias expression profiles associated with distinct IGH translocations in multiple myeloma

Data table header descriptions
ID_REF Affymetrix probes number ID
VALUE Average difference intensity (See Affymetrix Literature)
ABS_CALL The call in the absolute analysis that indicates if the transcript was present (P), absent (A) or marginal (M)
DETECTION P-VALUE the detection p-value in the absolute analysis used to assign the call present (P), absent (A) or marginal (M)

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 69.5 P 0.004017
AFFX-BioB-M_at 221 P 0.000446
AFFX-BioB-3_at 62.7 P 0.033677
AFFX-BioC-5_at 234.8 P 0.000195
AFFX-BioC-3_at 159.2 P 0.000225
AFFX-BioDn-5_at 226.6 P 0.001248
AFFX-BioDn-3_at 1422.7 P 0.000127
AFFX-CreX-5_at 2756.9 P 0.000044
AFFX-CreX-3_at 4040.1 P 0.000044
AFFX-DapX-5_at 11.1 A 0.139482
AFFX-DapX-M_at 22.2 A 0.156732
AFFX-DapX-3_at 3.9 A 0.876428
AFFX-LysX-5_at 1.9 A 0.737173
AFFX-LysX-M_at 11.8 A 0.58862
AFFX-LysX-3_at 16.2 A 0.250796
AFFX-PheX-5_at 1.8 A 0.910522
AFFX-PheX-M_at 1.5 A 0.904333
AFFX-PheX-3_at 4.2 A 0.883887
AFFX-ThrX-5_at 4.4 A 0.860518
AFFX-ThrX-M_at 3.4 A 0.712257

Total number of rows: 22283

Table truncated, full table size 578 Kbytes.




Supplementary file Size Download File type/resource
GSM38169.CEL.gz 3.3 Mb (ftp)(http) CEL

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