Barley (Hordeum vulgare L. cv. Graphic) seeds were carefully chosen with similar weight, shape and size and sown in 1 L pots containing sand in a glasshouse for 15 days (Experimental Fields of the University of Barcelona) and irrigated with a half strength Hoagland solution containing either 0 µM (control), 500 µM or 1000 µM of HgCl2. Daily means of temperature, relative humidity and maximum irradiance in the glasshouse throughout the experiment were 16ºC, 45% and 378 Wm-2, respectively. For root and shoot dry weight, 5 plantlets were sampled, immediately frozen in liquid nitrogen, lyophylized and dry weights were recorded and shoot to root ratio calculated. Additional three plantlets per treatment (only controls and 1000 μM HgCl2) were sampled and the entire roots were separated from leaves, washed with de-ionized water and immediately frozen in liquid N and kept at -80ºC until use for RNA extractions.
Extracted molecule
total RNA
Extraction protocol
Trizol reagent and Rneasy Quiagen
Label
biotin
Label protocol
Standard Affymetrix procedures.
Hybridization protocol
Standard Affymetrix procedures.
Scan protocol
Standard Affymetrix procedures.
Description
All sample extraction and micro chip manipulation were carried out at PROGENIKA, in SPAIN (Bilbao)
Data processing
Normalization was carried out in 2 steps: using GeneSpring v7.1 (Agilent) and previously scaled with GCOS.
Submission date
Mar 19, 2009
Last update date
May 12, 2009
Contact name
Marta Silva Lopes
Fax
525558047558
Organization name
cimmyt
Department
wheat physiology
Street address
km 45, carretera Mexico-Veracruz, El Batan, Texcoco
