|
| Status |
Public on Jun 06, 2019 |
| Title |
AAGATT |
| Sample type |
SRA |
| |
|
| Source name |
C57BL/6
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: CD19;L-gp
tissue: Tumor
phenotype: mature
percent_infiltration: 0
purified: Y
transplantation: N
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy plus mini Kit.
Generation of barcoded cDNA using Maxima RT polymerase. Application of template switch oligo. Library preparation with Nextera XT kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
R1 fastq file: cDNA; R2 fastq file: barcode/UMI
|
| Data processing |
Dropseq (v1.0) – see Macosko et al., PMID: 26000488.
Genome_build: GRCm38
Supplementary_files_format_and_content: Lgp130-Bcell-RawCounts.txt: Tab-delimited text file includes raw read counts for each sample.
Supplementary_files_format_and_content: Lgp130-Bcell-Eset.rds: ExpressionSet containing raw counts and metadata.
|
| |
|
| Submission date |
Jun 03, 2019 |
| Last update date |
Jun 06, 2019 |
| Contact name |
Hans Carlo Maurer |
| Organization name |
Klinikum rechts der Isar
|
| Department |
II. Medizinische Klinik
|
| Street address |
Ismaninger Str. 22
|
| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE132148 |
Gene expression from mouse models of B cell lymphomagenesis driven by gp130 signaling |
|
| Relations |
| BioSample |
SAMN11950890 |
| SRA |
SRX5964215 |
