|
| Status |
Public on Mar 24, 2009 |
| Title |
WT_Mstn_3 |
| Sample type |
RNA |
| |
|
| Source name |
gastrocnemius muscle
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
tissue: gastrocnemius muscle
age: 7.5 months
genotype: wild-type
|
| Treatment protocol |
All mice received a series of 8 injections of tamoxifen starting at 4 months of age to activate Cre recombinase to knock out myostatin in mice with floxed myostatin genes.
|
| Growth protocol |
Mice were reared under normal conditions with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted into Trizol as recommended by supplier (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared with kits from NuGEN as recommended by supplier.
|
| |
|
| Hybridization protocol |
Hybridization, washes, and staining with phycoerythrin-streptavidin (including antibody enhancement step) were carried out as recommended by Affymetrix for eukaryotic expression arrays, using hybridization oven and fluidics station purchased from Affymetrix.
|
| Scan protocol |
Scans were done with Affymetrix scanner exactly as recommended by Affymetrix.
|
| Description |
male, 7.5 months old, normal myostatin genes, CAGG-CreER transgene, C57BL/6 background
|
| Data processing |
background subtraction and normalization were done with the GCRMA method.
|
| |
|
| Submission date |
Mar 23, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Stephen Welle |
| E-mail(s) |
swelle@rochester.rr.com
|
| Organization name |
University of Rochester
|
| Street address |
601 Elmwood Avenue
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE15349 |
Skeletal muscle gene expression after myostatin knockout in mature mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
