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Sample GSM387071 Query DataSets for GSM387071
Status Public on Jan 01, 2010
Title AML with normal karyotype #12
Sample type RNA
Source name mononuclear cells from bone marrow
Organism Homo sapiens
Characteristics npm1: positive
flt3-itd: negative
cebpa: negative
age: 50
diagnosis: AML with normal karyotype
gender: male
specimen type: Bone Marrow
blast cell percentage: 71
disease state: de novo AML
Treatment protocol Samples are from untreated patients (de novo AML)
Extracted molecule total RNA
Extraction protocol The total RNA was purified either with Qiagen RNeasy Mini kits (Qiagen, Hilden, Germany) or with TRIzol-based protocols.
Label Biotin
Label protocol For each sample preparation, total RNA was converted into double-stranded cDNA by reverse transcription using a cDNA Synthesis System kit including an oligo(dT)24 – T7 primer (Roche Applied Science, Mannheim, Germany) and Poly-A control transcripts (Affymetrix, Santa Clara, CA, USA). The generated cRNA was purified using the GeneChip Sample Cleanup Module (Affymetrix) and quantified using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). The incubation steps during the cDNA synthesis, in vitro transcription reaction, and target fragmentation were performed using the Hybex Microarray Incubation System (SciGene, Sunnyvale, CA, USA) and Eppendorf ThermoStat plus instruments (Eppendorf, Hamburg, Germany).
Hybridization protocol Hybridization, washing, and staining protocols, respectively, were performed on Affymetrix GeneChip instruments (Hybridization Oven 640, Fluidics Station FS450) as recommended by the manufacturer.
Scan protocol Scanning was performed on Affymetrix GeneChip Scanner GCS3000 instruments as recommended by the manufacturer using default settings. The software used was GCOS 1.2 or higher.
Description DRE
Data processing The Robust Multichip Average (RMA) algorithm as implemented in the R package affy (version 1.20.2) was applied to generate probe set level signal intensities. (Irizarry et al., Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 2003 Apr;4(2):249-64)
Submission date Mar 27, 2009
Last update date Nov 14, 2018
Contact name Hans-Ulrich Klein
Organization name Columbia University Medical Center
Department Neurology
Lab Center for Translational and Computational Neuroimmunology
Street address 622 W 168th St
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platform ID GPL570
Series (1)
GSE15434 Gene expression profiling in AML with normal karyotype: A multicenter study investigating molecular markers in 251 cases
Reanalyzed by GSE119087
Reanalyzed by GSE122511

Data table header descriptions
VALUE The Robust Multichip Average (RMA) expression measure

Data table
1007_s_at 3.909126965
1053_at 5.482930578
117_at 6.177784426
121_at 7.191586786
1255_g_at 2.11980325
1294_at 7.495423487
1316_at 5.029664205
1320_at 2.533988721
1405_i_at 8.946655433
1431_at 2.332814271
1438_at 3.1195381
1487_at 6.372938245
1494_f_at 3.986775187
1552256_a_at 5.690385739
1552257_a_at 6.348715244
1552258_at 3.960669334
1552261_at 3.199210852
1552263_at 7.05278868
1552264_a_at 7.867920695
1552266_at 2.702447088

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.

Supplementary file Size Download File type/resource
GSM387071.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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