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Sample GSM388024 Query DataSets for GSM388024
Status Public on Oct 07, 2009
Title SBA_30
Sample type genomic
 
Channel 1
Source name small intestinal adenocarcinoma
Organism Homo sapiens
Characteristics age: 69
sex: male
tumor site: Jejunum
celiac disease: No
tnm: pT3N1Mx
platform_id type: oligo
Extracted molecule genomic DNA
Extraction protocol DNA extraction was performed using the the QIAmp DNA Micro Kit (Qiagen).
Label Cy3
Label protocol According Van den IJssel et al, Nucleic Acids Res. 2005 Dec 16;33(22):e192
 
Channel 2
Source name female reference DNA
Organism Homo sapiens
Characteristics sample type: Pooled DNA from the blood of 10 normal female individuals
Extracted molecule genomic DNA
Extraction protocol DNAzol (Invitrogen) according to manufacturer's protocol
Label Cy5
Label protocol According Van den IJssel et al, Nucleic Acids Res. 2005 Dec 16;33(22):e192
 
 
Hybridization protocol According Van den IJssel et al, Nucleic Acids Res. 2005 Dec 16;33(22):e192
Scan protocol Microarray Scanner G2505B (Agilent Technologies), default settings
Description 600ng of tumor and normal DNA were combined with 10 µg Cot-1 DNA and precipitated by adding 2.5 volumes of ice-cold 100% ethanol and 0.1 volume of 3M sodium acetate (pH 5.2). The DNA was collected by centrifugation at 14000 rpm for 30 minutes at 4 °C. The pellet was dissolved in 130 µl hybridization solution and incubated at 73°C for 10 minutes to denature the DNA, followed by 60-120 minute incubation at 37°C. Next, the hybridization mixture was added to the array in a hybridization station (HybArray12TM, Perkin Elmer Life Sciences, Zaventum, Belgium) and incubated for 38 hours at 37°C. After hybridization the slides were washed in six washing steps and dried by centrifugation for 3 minutes at 1000 g.
Data processing The raw signal intensities were obtained with Bluefuse. The unreliable and morphologically corrupted measurement spots were removed from the data. We used the global mode normalization.
 
Submission date Mar 31, 2009
Last update date Aug 09, 2010
Contact name Daoud Sie
E-mail(s) d.sie@vumc.nl
Phone +31 20 4442428
Organization name Vrije Universiteit Medical Center
Department Pathology
Lab Microarray Core Facility
Street address De Boelelaan 1117
City Amsterdam
ZIP/Postal code 1081 HV
Country Netherlands
 
Platform ID GPL8368
Series (2)
GSE15470 Array comparative genomic hybridization in sporadic and coeliac disease-related small bowel adenocarcinomas
GSE23418 Small bowel adenocarcinoma copy number profiles are more closely related to colorectal than to gastric cancers

Data table header descriptions
ID_REF
AMPCH1 Total signal in channel 1 (Cy3)
AMPCH2 Total signal in channel 2 (Cy5)
LOG2RATIO CH1/CH2 Log, base 2, of the ratio of total signal in channel 1 divided by total signal in channel 2
CONFIDENCE The Confidence Estimate in the calculated ratio, between 0 and 1, calculated by BlueFuse
QUALITY A flag to highlight spots that suffer from poor printing and other experimental artefacts (1 acceptable; 0 not acceptable), estimated by BlueFuse
VALUE normalized log2 ratio (CH1/CH2) but with flagged values removed
UNF_VALUE normalized log2 ratios

Data table
ID_REF AMPCH1 AMPCH2 LOG2RATIO CH1/CH2 CONFIDENCE QUALITY VALUE UNF_VALUE
1 236.16 139.347 0.761 0.6 0
2 150.765 100.508 0.585 0.78 0
3 138.051 125.817 0.134 0.86 0
4 1320.767 1141.98 0.21 0.97 0
5 2203.451 2060.646 0.097 0.99 0
6 979.65 1071.845 -0.13 0.97 0
7 112.261 175.989 -0.649 0.81 1 -0.209 -0.209
8 818.255 1114.419 -0.446 0.98 1 -0.006 -0.006
9 101.149 146.71 -0.536 0.75 1 -0.096 -0.096
10 308.07 406.707 -0.401 0.97 1 0.039 0.039
11 160.323 266.976 -0.736 0.92 1 -0.296 -0.296
12 511.932 651.915 -0.349 0.98 1 0.091 0.091
13 146.214 206.6 -0.499 0.91 1 -0.059 -0.059
14 174.059 375.283 -1.108 0.94 1 -0.668 -0.668
15 250.904 440.643 -0.812 0.97 1 -0.372 -0.372
16 173.362 258.662 -0.577 0.94 1 -0.137 -0.137
17 352.321 417.204 -0.244 0.97 1 0.196 0.196
18 410.428 485.532 -0.242 0.96 1 0.198 0.198
19 124.479 214.435 -0.785 0.91 1 -0.345 -0.345
20 154.739 247.805 -0.679 0.93 1 -0.239 -0.239

Total number of rows: 30000

Table truncated, full table size 1339 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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