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Sample GSM3881033 Query DataSets for GSM3881033
Status Public on Sep 05, 2019
Title 2KD_CIS
Sample type SRA
Source name xenograft ovarian tumor from mouse
Organism Homo sapiens
Characteristics cell line: A2780
transfection: shRNA targeting GDF15
host strain: Swiss nude mouse
age (weeks): 8-10 weeks
treatment: mouse treated with cisplatin
Extracted molecule total RNA
Extraction protocol Total RNA were extracted from A2780 xenograft mouse tumors that have been stored in RNAlater solution using Qiagen RNeasy Mini Kit.
Sequencing libraries were prepared using KAPA Stranded mRNA seq Kit and run in a single lane using Illumina HiSeq 4000 instrument.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were processed with RNA-seq workflow in CLC Genomics Workbench version 11. Reads were mapped to human reference genome GRCh38 with default setting.
Gene expression is estimated with the EM estimation algorithm and reported as TPM (Transcripts Per Million)
Genome_build: GRCh38
Supplementary_files_format_and_content: Excel files with gene expression data in TPM and RPKM
Submission date Jun 12, 2019
Last update date Sep 05, 2019
Contact name Kwong-Kwok Wong
Phone 713-792-0229
Organization name UT M. D. Anderson Cancer Center
Department Gynecologic Oncology and Reproductive Medicine
Street address T4-3900, 1515 Holcombe Boulevard
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platform ID GPL20301
Series (1)
GSE132610 Impact of GDF15 expression of ovarian cancer cell on stroma and Cisplatin responses
BioSample SAMN12040489
SRA SRX6052943

Supplementary file Size Download File type/resource
GSM3881033_2KD_CIS.xlsx 8.5 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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