|
| Status |
Public on Jul 16, 2009 |
| Title |
HPDE |
| Sample type |
RNA |
| |
|
| Source name |
Normal Pancreatic cell line, HPDE
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HPDE
cell type: pancreatic ductal epithelial
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol reagent (Molecular lab), followed by clean up with RNeasy® Mini kit (Qiagen, Valencia, CA). RNA quality and integrity were confirmed by Bioanalyzer (Agilent technology)
|
| Label |
biotin
|
| Label protocol |
cRNA samples were prepared for hybridization according to Illumina's instructions: 500 ng of total RNA from each sample was converted to cDNA synthesis then to biotin labeled cRNA by in vitro transcription using the Illumina TotalPrep RNA Amplification kit (Ambion Inc., Austin, TX). cRNA concentrations were measured using spectrophotometer (Nanodrop, ND-1000) and cRNA size was measured using Agilent bioanalyzer.
|
| |
|
| Hybridization protocol |
1.5 ug/array of biotin-labled cRNA was hybridized to Illumina Human-6 v 2 (48K) according to the Manufacturer's instructions (Illumina, Inc., San Diego, CA). Amersham fluorolink streptavidin-Cy3 (GE Healthcare Bio-Sciences, Little Chalfont, UK) was used to develop the array signal.
|
| Scan protocol |
BeadStation 500GXDW (Illumina, Inc., San Diego, CA) was used to scan array chips.
|
| Description |
Illumina Human-6 v 2 has 46713 probes.
|
| Data processing |
Signal intensities were quantified with Illumina BeadStudio software.
|
| |
|
| Submission date |
Apr 03, 2009 |
| Last update date |
Jul 16, 2009 |
| Contact name |
Woonyoung Choi |
| Organization name |
Md Anderson Cancer center
|
| Street address |
1515 Holcombe
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6790 |
| Series (1) |
| GSE15550 |
Gene expression of pancreatic cancer cells |
|
