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Sample GSM3903978 Query DataSets for GSM3903978
Status Public on Jun 25, 2019
Title HC 88
Sample type protein
 
Source name Culture supernatant from Mtb-stimulated whole blood
Organism Homo sapiens
Characteristics tissue: Whole blood
histology: Healthy
Treatment protocol Whole blood (250 µl) collected from participants using an EDTA anticoagulation tube was cultured in serum-free medium (DKW34-EHK0100, DAKEWE) in absence or presence of 2.5 µg/ml phytohemagglutinin (PHA, L8754, Sigma) or 10 µg/ml Mtb-antigen peptide pool for 24 h at 37°C, 5% CO2, and then the supernatant was collected.
Extracted molecule protein
Extraction protocol Culture supernatant from Mtb-stimulated whole blood
Label AlexaFluor 555
Label protocol AlexaFluor 555-conjugated streptavidin was then added and incubated for 1 hour at room temperature.
 
Hybridization protocol 2-fold diluted culture supernatant was added to each well. After overnight incubation at 4°C and extensive washing, the biotin-labeled detection antibody was added for 2 hours and then washed away. AlexaFluor 555-conjugated streptavidin was then added and incubated for 1 hour at room temperature.
Scan protocol The signals (532 nm excitation, 635 nm emission) were scanned and extracted using an InnoScan 300 scanner (Innopsys, Carbonne, France).
Description A combination of 16 non-overlapping arrays to quantitatively measure 640 human cytokines. Each antibody, together with the positive controls is arrayed in quadruplicate.
Data processing Individual array spots were background subtracted locally by subtracting the median background across spot replicated in each sample, and then normalized using two positive controls. For protein quantification, standard curves were generated using purified proteins representing the proteins-of-interest.
 
Submission date Jun 24, 2019
Last update date Jun 25, 2019
Contact name Huihua Zhang
Organization name Jinan University
Department Biomedicine Institute & Cell Biology Department
Street address 601, west Huangpu avenue, Tianhe district
City Guangzhou
ZIP/Postal code 510632
Country China
 
Platform ID GPL26838
Series (1)
GSE133249 Identification of an eight-protein biosignature for the diagnosis of tuberculosis

Data table header descriptions
ID_REF
VALUE All the raw data were log2-transformed for statistical analysis.

Data table
ID_REF VALUE
1 12.91954865
2 10.77829072
3 14.07491246
4 8.759921463
5 7.800770504
6 11.18905236
7 16.3839248
8 6.887647115
9 12.31080872
10 9.941634545
11 10.89150273
12 9.61845892
13 12.94298543
14 11.35222554
15 17.1010147
16 8.295126063
17 10.17058859
18 10.82518141
19 10.25187292
20 9.590437346

Total number of rows: 640

Table truncated, full table size 9 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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