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Sample GSM3908387 Query DataSets for GSM3908387
Status Public on Dec 31, 2021
Title G1E-ER4_12.5nM_rep2
Sample type SRA
 
Source name cell line
Organism Mus musculus
Characteristics cell line: G1E-ER4
agent: beta estradiol
agent amount: 12.5nM
Treatment protocol indicated concentrations of beta-estradiol
Growth protocol IMDM, 15% FBS, 1xPen/Strep, 4.5E-5M mono-thio-glycerate, 50ng/ml murine SCF and 2U/ml erythropoietin
Extracted molecule total RNA
Extraction protocol Qiagen RLT lysis buffer
Smart-seq2
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Raw sequencing data was processed using bcl2fastq
Paired-end reads were aligned to the mm10 reference genome using STAR.
Gene count abundances were estimated using the geneCounts flag with STAR
NA
Genome_build: mm10
Supplementary_files_format_and_content: Per-sample raw gene counts matrix
 
Submission date Jun 27, 2019
Last update date Dec 31, 2021
Contact name Caleb Lareau
E-mail(s) lareauc@mskcc.org
Organization name Memorial Sloan Kettering
Street address 417 E 68th St, Zuckerman - ZRC 1132
City New York
State/province New York
ZIP/Postal code 10065
Country USA
 
Platform ID GPL19057
Series (2)
GSE133415 A Congenital Anemia Dissociates the Pleiotropic Functions of Master Transcription Factor GATA1 [Beta-estradiol_RNAseq]
GSE133417 A Congenital Anemia Dissociates the Pleiotropic Functions of Master Transcription Factor GATA1
Relations
BioSample SAMN12147697
SRA SRX6370232

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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