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Sample GSM3931439 Query DataSets for GSM3931439
Status Public on Aug 08, 2021
Title A549_siNT_rep2 RNA-Seq
Sample type SRA
 
Source name A549
Organism Homo sapiens
Characteristics cell type: Lung adenocarcinoma
treatment: siNT
Treatment protocol DMSO or 10 ┬ÁM of GSK-J4, and siNT or siKDM6B were treated for 24hr.
Growth protocol HCC827, H23 and A549 were cultured in DMEM (Welgene, Republic of Korea) supplemented with 10% fetal bovine serum (Welgene, Republic of Korea).
Extracted molecule total RNA
Extraction protocol Total RNAs were isolated using RNeasy mini kits (Qiagen, Valencia, CA).
The Libraries were prepared using the TruSeq stranded mRNA sample preparation kit (Illumina).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Data processing Raw reads were mapped to the reference human (hg19) genome assembly with Tophat, and rpkm values were calculated with Cufflink
Genome_build: hg19
Supplementary_files_format_and_content: none
 
Submission date Jul 08, 2019
Last update date Aug 08, 2021
Contact name Mi Young Kim
E-mail(s) miyoungkim@kaist.ac.kr
Organization name KAIST
Street address Biological science
City DaeJeon
ZIP/Postal code 305-701
Country South Korea
 
Platform ID GPL18573
Series (2)
GSE118319 Genome-wide RNA-seq from GSK-J4-treated lung adenocarcinoma cell lines
GSE133970 Genome-wide assessment of GSK-J4-treated lung adenocarcinoma cell lines
Relations
BioSample SAMN12232124
SRA SRX6415620

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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