|
| Status |
Public on Aug 08, 2021 |
| Title |
A549_siKDM6B_rep1 RNA-Seq |
| Sample type |
SRA |
| |
|
| Source name |
A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Lung adenocarcinoma
treatment: siKDM6B
|
| Treatment protocol |
DMSO or 10 µM of GSK-J4, and siNT or siKDM6B were treated for 24hr.
|
| Growth protocol |
HCC827, H23 and A549 were cultured in DMEM (Welgene, Republic of Korea) supplemented with 10% fetal bovine serum (Welgene, Republic of Korea).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated using RNeasy mini kits (Qiagen, Valencia, CA).
The Libraries were prepared using the TruSeq stranded mRNA sample preparation kit (Illumina).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Raw reads were mapped to the reference human (hg19) genome assembly with Tophat, and rpkm values were calculated with Cufflink
Genome_build: hg19
Supplementary_files_format_and_content: none
|
| |
|
| Submission date |
Jul 08, 2019 |
| Last update date |
Aug 08, 2021 |
| Contact name |
Mi Young Kim |
| E-mail(s) |
miyoungkim@kaist.ac.kr
|
| Organization name |
KAIST
|
| Street address |
Biological science
|
| City |
DaeJeon |
| ZIP/Postal code |
305-701 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE118319 |
Genome-wide RNA-seq from GSK-J4-treated lung adenocarcinoma cell lines |
| GSE133970 |
Genome-wide assessment of GSK-J4-treated lung adenocarcinoma cell lines |
|
| Relations |
| BioSample |
SAMN12232123 |
| SRA |
SRX6415621 |
