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Status |
Public on Aug 08, 2021 |
Title |
H23_siNT_rep1 RNA-Seq |
Sample type |
SRA |
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Source name |
H23
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Organism |
Homo sapiens |
Characteristics |
cell type: Lung adenocarcinoma treatment: siNT
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Treatment protocol |
DMSO or 10 µM of GSK-J4, and siNT or siKDM6B were treated for 24hr.
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Growth protocol |
HCC827, H23 and A549 were cultured in DMEM (Welgene, Republic of Korea) supplemented with 10% fetal bovine serum (Welgene, Republic of Korea).
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were isolated using RNeasy mini kits (Qiagen, Valencia, CA). The Libraries were prepared using the TruSeq stranded mRNA sample preparation kit (Illumina).
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
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Data processing |
Raw reads were mapped to the reference human (hg19) genome assembly with Tophat, and rpkm values were calculated with Cufflink Genome_build: hg19 Supplementary_files_format_and_content: none
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Submission date |
Jul 08, 2019 |
Last update date |
Aug 08, 2021 |
Contact name |
Mi Young Kim |
E-mail(s) |
miyoungkim@kaist.ac.kr
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Organization name |
KAIST
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Street address |
Biological science
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City |
DaeJeon |
ZIP/Postal code |
305-701 |
Country |
South Korea |
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Platform ID |
GPL18573 |
Series (2) |
GSE118319 |
Genome-wide RNA-seq from GSK-J4-treated lung adenocarcinoma cell lines |
GSE133970 |
Genome-wide assessment of GSK-J4-treated lung adenocarcinoma cell lines |
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Relations |
BioSample |
SAMN12232121 |
SRA |
SRX6415623 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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