|
| Status |
Public on Jan 04, 2010 |
| Title |
Drosophila OSS cells_1 |
| Sample type |
RNA |
| |
|
| Source name |
OSS cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: OSS
cell type: follicular stem cell
|
| Biomaterial provider |
Yuzo Niki supplied original starting culture, while cultures utilized in these experiments were grown by Nelson Lau.
|
| Treatment protocol |
Cells grown in exponential phase in standard medium, no additional treatment
|
| Growth protocol |
Cells were cultured in 6 well plates, split every 3 days 1:3, and were cultured in Shield and Sang's M3 media supplemented with 10% fetal bovine serum, 1ug/ml insulin, 60ug/ml glutathione, and 5% fly extract.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Near-confluent wells washed once with 1XPBS, then subject to Trizol extraction to obtain total RNA.
|
| Label |
Biotin
|
| Label protocol |
10 ug of total RNA from each sample was converted to cDNA and then to biotinylated cRNA with the One-Cycle Target Labeling protocol as detailed in the Affymetrix manual for GeneChip® Eukaryotic Labeling Assays for Expression Analysis.
|
| |
|
| Hybridization protocol |
Hybridization on the Affymetrix Fluidics 450 system was performed with the standard protocol #FS450_0002 as detailed in the program software.
|
| Scan protocol |
Scanning was performed on the GeneChip ® Scanner 3000 using the preset pixel value and resolution parameters defined in the scanner.
|
| Description |
OSS cells are a stable cell line derived from follicular stem cells of the Drosophila ovary. The cells express the Fas III gene as a marker of follicle cells, and are described in Niki et. al., Proc Natl Acad Sci USA 2006 Oct 31;103(44): 16325-30.
CEL and DAT files were outputed from scanning and utilized in custom analysis scripts
|
| Data processing |
Array files were processed with Bioconductor scripts in the R statistical and graphing package.
|
| |
|
| Submission date |
Apr 25, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Nicolas Robine |
| Organization name |
Sloan-Kettering Institute
|
| Department |
Developmental Biology
|
| Lab |
Dr. Eric Lai's Lab
|
| Street address |
430, East 67th Street, RRL 517
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE15825 |
An mRNA/3' UTR-directed primary piRNA pathway in Drosophila ovarian somatic cells |
|
| Relations |
| Reanalyzed by |
GSE119084 |
