|
| Status |
Public on May 13, 2009 |
| Title |
Day1-RT1-ArrayA |
| Sample type |
RNA |
| |
|
| Source name |
Proliferating C2C12 cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: muscle
strain: C3H
cell type: myoblast
time: day 1
time: aliquot 1
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
N/A
|
| Growth protocol |
Mouse C2C12 myoblasts were cultured in growth media containing Dulbecco's Modified Eagle Medium (DMEM) (ATCC), supplemented with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT) and 1% penicillin-streptomycin (Mediatech, Inc., Herndon, VA) at 37°C in a humidified atmosphere of 5% CO2 in air.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from proliferating C2C12 cells using TRIzol (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. Total RNA concentration was quantified by an absorbance at 260 nm using a SmartSpec 3000 spectrophotometer (Bio-Rad, Hercules, CA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and samples with RNA integrity number (RIN) >9 were used in the array studies. Aliquots of the same RNA sample were used for all the experiments in both the microarray and qPCR-array measurements.
|
| Label |
FAM
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
N/A
|
| Scan protocol |
N/A
|
| Description |
Standard Taqman Assay: Quantitative real-time PCR was performed using Applied Biosystems 7900HT system and universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles).
|
| Data processing |
Data was processed using SDS 2.3 software (Applied Biosystems, Foster City, CA). Normalized by subtracting mammu6 to get Delta CT
|
| |
|
| Submission date |
May 06, 2009 |
| Last update date |
May 12, 2009 |
| Contact name |
Jonathan Adam Gelfond |
| E-mail(s) |
gelfondjal@uthscsa.edu
|
| Phone |
210 567 0851
|
| Organization name |
UT Health Science Ctr San Antonio
|
| Department |
Epidemiology & Biostatistics
|
| Lab |
Bioinformatics
|
| Street address |
Mail Code 7933, 7703 Floyd Curl
|
| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78229 |
| Country |
USA |
| |
|
| Platform ID |
GPL8527 |
| Series (1) |
| GSE16000 |
Reproducibility of Quantitative RT-PCR Array in miRNA Expression Profiling and Comparison with Microarray Analysis |
|
