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Sample GSM400681 Query DataSets for GSM400681
Status Public on May 13, 2009
Title Amp2_ArrayB
Sample type RNA
 
Source name Proliferating C2C12 cells
Organism Mus musculus
Characteristics tissue: muscle
strain: C3H
cell type: myoblast
protocol: amplification
Biomaterial provider ATCC
Treatment protocol N/A
Growth protocol Mouse C2C12 myoblasts were cultured in growth media containing Dulbecco's Modified Eagle Medium (DMEM) (ATCC), supplemented with 10% fetal bovine serum (FBS) (Hyclone, Logan, UT) and 1% penicillin-streptomycin (Mediatech, Inc., Herndon, VA) at 37°C in a humidified atmosphere of 5% CO2 in air.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from proliferating C2C12 cells using TRIzol (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions. Total RNA concentration was quantified by an absorbance at 260 nm using a SmartSpec 3000 spectrophotometer (Bio-Rad, Hercules, CA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) and samples with RNA integrity number (RIN) >9 were used in the array studies. Aliquots of the same RNA sample were used for all the experiments in both the microarray and qPCR-array measurements.
Label FAM
Label protocol N/A
 
Hybridization protocol N/A
Scan protocol N/A
Description Standard Taqman Assay: Quantitative real-time PCR was performed using Applied Biosystems 7900HT system and universal cycling conditions (10 min. at 95C; 15 sec. at 95C; 1 min. at 60C for 40 cycles).
Data processing Data was processed using SDS 2.3 software (Applied Biosystems, Foster City, CA). Normalized by subtracting mammu6 to get Delta CT
 
Submission date May 07, 2009
Last update date Mar 10, 2011
Contact name Jonathan Adam Gelfond
E-mail(s) gelfondjal@uthscsa.edu
Phone 210 567 0851
Organization name UT Health Science Ctr San Antonio
Department Epidemiology & Biostatistics
Lab Bioinformatics
Street address Mail Code 7933, 7703 Floyd Curl
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
 
Platform ID GPL8526
Series (1)
GSE16000 Reproducibility of Quantitative RT-PCR Array in miRNA Expression Profiling and Comparison with Microarray Analysis

Data table header descriptions
ID_REF
VALUE Delta Ct value

Data table
ID_REF VALUE
ath-miR159a-4373390 25.988
MammU6-4395470
mmu-let-7a*-4395608 9.28
mmu-let-7b*-4395515 23.594
mmu-let-7c-1*-4395609 14.469
mmu-let-7d*-4378108 17.286
mmu-let-7f*-4395528 17.231
mmu-let-7g*-4395622 18.607
mmu-let-7i*-4395283 17.418
mmu-miR-101a*-4395637 25.988
mmu-miR-101b-4395661 9.126
mmu-miR-106b*-4395491 10.106
mmu-miR-10a*-4395399 20.309
mmu-miR-10b*-4395702 25.988
mmu-miR-124*-4395308 25.988
mmu-miR-125b*-4395638 15.652
mmu-miR-127*-4395340 25.988
mmu-miR-130b*-4395590 11.972
mmu-miR-133a*-4381060 25.988
mmu-miR-135a*-4395343 8.464

Total number of rows: 216

Table truncated, full table size 5 Kbytes.




Supplementary file Size Download File type/resource
GSM400681.txt.gz 1.8 Kb (ftp)(http) TXT
Processed data included within Sample table

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