|
| Status |
Public on May 12, 2009 |
| Title |
Toxoplasma gondii Type III CTGara Compound 1 - 2 |
| Sample type |
RNA |
| |
|
| Source name |
Type III CTGara Compound 1 48hrs
|
| Organism |
Toxoplasma gondii |
| Characteristics |
strain: type III strain CTGara
developmental stage: bradyzoite
growth condition: grown in human foreskin fibroblasts under 3uM Compound 1 for 48hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Parasites were purified from host cells and RNA was extracted using the Qiagen RNeasy kit (b-mercaptoethanol and DNase I treatment).
|
| Label |
biotin
|
| Label protocol |
Affymetrix One Cycle: 3ug starting RNA - Standard One Cycle protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol with 5ug labeled cRNA.
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Type III strain CTGara grown in human foreskin fibroblasts under 3uM Compound 1 for 48hrs.
For additional Material and Methods see:
Mol Microbiol. 2008 Jun;68(6):1502-18. Epub 2008 Apr 21.
The transcription of bradyzoite genes in Toxoplasma gondii is controlled byautonomous promoter elements.
Behnke MS, Radke JB, Smith AT, Sullivan WJ Jr, White MW.
|
| Data processing |
GeneSpring 7.2 RMA, Data transformation: Set measurements less than 0.01 to 0.01, Per Chip and Per Gene: Median Polishing
|
| |
|
| Submission date |
May 10, 2009 |
| Last update date |
Mar 04, 2010 |
| Contact name |
Michael Sean Behnke |
| E-mail(s) |
mike_behnke@yahoo.com
|
| Organization name |
Washington University School of Medicine
|
| Department |
Molecular Microbiology
|
| Lab |
Dr David Sibley
|
| Street address |
660 S. Euclid Ave
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL7186 |
| Series (1) |
| GSE16037 |
Types I GT1, II Me49, III CTGara strains - tachyzoite and Compound 1 |
|
