|
| Status |
Public on Dec 31, 2009 |
| Title |
Sarcomatoid_2 |
| Sample type |
RNA |
| |
|
| Source name |
F1 from Fischer344 and Brown-Norway, male, ferric saccharate induced mesothlioma of sarcomatoid types
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: F1 from Fischer344 and Brown-Norway
gender: female
tissue: mesothlioma of sarcomatoid types
sample: tumor
|
| Treatment protocol |
ferric saccharate induced
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy Mini(QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008).
|
| |
|
| Hybridization protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008).
|
| Scan protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008, Agilent Scanner Settings).
|
| Description |
Original sample number: FB12-4
|
| Data processing |
Microarray data were analyzed following import into GeneSpring GX 10.0.2 software (Agilent Technologies)
Threshold: 1.0
Logbase: 2
Technology: Agilent.SingleColor.14879
Normalization: Shift to 75 percentile
Baseline Transformation: Median of Control Samples
|
| |
|
| Submission date |
May 14, 2009 |
| Last update date |
May 21, 2009 |
| Contact name |
Shinya Toyokuni |
| E-mail(s) |
akatsuka@med.nagoya-u.ac.jp
|
| Organization name |
Nagoya University
|
| Department |
Pathology
|
| Street address |
65 Tsuruma-Cho, Showa-Ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7294 |
| Series (1) |
| GSE16138 |
Transcriptome analysis of mesothelioma induced by intraperitoneal injections of ferric saccharate in rats |
|
