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Sample GSM4037768 Query DataSets for GSM4037768
Status Public on Mar 19, 2021
Title Wt_M_DRG3
Sample type SRA
Source name DRG tissues from naïve males
Organism Mus musculus
Characteristics strain: C57BL
age: 3-4 month
Sex: Male
Extracted molecule total RNA
Extraction protocol Single cell suspension from TG and DRG tissues. Sensory neuron fraction was isolated by using Percoll gradient approach
Approximately 10-20ng Total RNA was used for RNA-seq library preparation by following kapa rRNA depletion Total RNA-se sample preparation kit
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Data processing Illumina bcl2fastq software used for base-calling
All RNA-seq Fastq reads were aligned with the reference genome (UCSC mouse genome build mm9) using TopHat2 default settings (Trapnell et al., 2012).
The BAM files obtained after alignment were processed using htseq-count to obtain the counts per gene in all samples (Anders et al., 2015).
Genome_build: UCSC mouse genome build mm9
Supplementary_files_format_and_content: The processed data files are in tabular format. The first column contains the gene symbols and the second column contain the read counts of the gene using htseq-count
Submission date Aug 16, 2019
Last update date Mar 19, 2021
Contact name YI ZOU
Organization name UTHSA-GCCRI
Street address 8403 Floyd Curl Drive
State/province TX
ZIP/Postal code 78229
Country USA
Platform ID GPL21493
Series (1)
GSE135909 DRG (dorsal root ganglia) and TG (trigeminal ganglia) fractions enriched with sensory neurons
BioSample SAMN12588301
SRA SRX6727681

Supplementary file Size Download File type/resource
GSM4037768_Wt_M_DRG3_Genes_ReadCount.txt.gz 92.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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