|
| Status |
Public on Jun 15, 2009 |
| Title |
DNA Pol2 asyn 136 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Input DNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: haploid W303a cells
|
| Growth protocol |
W303 DNA Pol2-13MYC tagged asynchronous cells grown in YEPD rich media to OD of 0.25, fixed, and genomic DNA was purified after reversing crosslinks
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Fixed cells were washed and lysed using zymolyase, sonicated to shear chromatin, and DNA was isolated
|
| Label |
Cy3
|
| Label protocol |
Input DNA was labeled using Cy3
|
| |
|
| Channel 2 |
| Source name |
ChIP DNA IP using MYC antibody
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: DNA Pol2-MYC expressing W303a haploid cells
|
| Growth protocol |
W303 DNA Pol2-13MYC tagged asynchronous cells grown in YEPD rich media to OD of 0.25, fixed
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Fixed cells were washed and lysed using zymolyase, sonicated to shear chromatin, and extract was used to IP DNA Pol2-13MYC using MYC Ab.
|
| Label |
Cy5
|
| Label protocol |
IP'ed DNA was labelled with Cy5
|
| |
|
| |
| Hybridization protocol |
Microarray hybridizations were performed as previously described (Iyer et al., 2001) at 65ºC for 12 to 16 hours.
|
| Scan protocol |
Fluorescent array images were collected for both Cy3 and Cy5 with a GenePix 4000B fluorescent scanner and image intensity data were extracted and analyzed with using Genepix 6.1 software.
|
| Description |
DNA Pol2-MYC ChIP-chip from W303a asynchronous cells
|
| Data processing |
After background correction and removal of flagged values, log base 2 expression ratios were normalized to the median value
|
| |
|
| Submission date |
May 21, 2009 |
| Last update date |
May 27, 2009 |
| Contact name |
Anna Azvolinsky |
| E-mail(s) |
aazvolin@gmail.com
|
| Organization name |
Princeton University
|
| Department |
Molecular Biology
|
| Lab |
Zakian Lab
|
| Street address |
Washington Road
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08540 |
| Country |
USA |
| |
|
| Platform ID |
GPL8192 |
| Series (1) |
| GSE16218 |
Mapping Replication Fork Pause Sites in S. cerevisiae |
|
