|
| Status |
Public on Mar 26, 2021 |
| Title |
Biopsy patient2 lesional skin day 0 |
| Sample type |
RNA |
| |
|
| Source name |
Lesional skin punch biopsy day 0
|
| Organism |
Homo sapiens |
| Characteristics |
patient diagnosis: psoriasis
tissue: skin
gender: Male
disease state (ls/nl): LS
day: 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from skin biopsies was isolated using the SV Total RNA Isolation System according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the protocol for the human Clariom D assay (ThermoFisher Scientific).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the human Clariom D GeneChip according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 system.
|
| Description |
Gene expression data from lesional psoriatic skin at day 0
|
| Data processing |
The microarray data were summarized applying the SST-RMA (signal space transformation - robust multi-array average) gene level method as implemented in the Transcriptome Analysis Console (TAC) 4.0 software (ThermoFisher Scientific).
|
| |
|
| Submission date |
Sep 10, 2019 |
| Last update date |
Mar 27, 2021 |
| Contact name |
Thomas Litman |
| E-mail(s) |
tlitman@hotmail.com
|
| Phone |
4541851753
|
| Organization name |
University of Copenhagen
|
| Department |
ISIM
|
| Street address |
Blegdamsvej 3C
|
| City |
Copenhagen N |
| State/province |
Outside the US or Canada |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL23126 |
| Series (2) |
| GSE137218 |
Skin expression data from psoriasis patients treated with secukinumab |
| GSE137221 |
Skin and blood expression data from psoriasis patients treated with secukinumab |
|
