|
| Status |
Public on Mar 26, 2021 |
| Title |
Blood sample patient3 day 0 |
| Sample type |
RNA |
| |
|
| Source name |
Blood sample day 0
|
| Organism |
Homo sapiens |
| Characteristics |
patient diagnosis: psoriasis
tissue: blood
gender: Female
day: 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMCs were isolated from blood samples taken from psoriatic patients and then total RNA from the PBMCs was isolated using the SV Total RNA Isolation System according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the protocol for the human Clariom D assay (ThermoFisher Scientific).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on the human Clariom D GeneChip according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 system.
|
| Description |
Gene expression data from isolated PBMCs from blood samples from psoriatic patients day 0
|
| Data processing |
The microarray data were summarized applying the SST-RMA (signal space transformation - robust multi-array average) gene level method as implemented in the Transcriptome Analysis Console (TAC) 4.0 software (ThermoFisher Scientific).
|
| |
|
| Submission date |
Sep 10, 2019 |
| Last update date |
Mar 27, 2021 |
| Contact name |
Thomas Litman |
| E-mail(s) |
tlitman@hotmail.com
|
| Phone |
4541851753
|
| Organization name |
University of Copenhagen
|
| Department |
ISIM
|
| Street address |
Blegdamsvej 3C
|
| City |
Copenhagen N |
| State/province |
Outside the US or Canada |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL23126 |
| Series (2) |
| GSE137220 |
Blood expression data from psoriasis patients treated with secukinumab |
| GSE137221 |
Skin and blood expression data from psoriasis patients treated with secukinumab |
|
